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Objective:To investigate the relation between Shp-2 (a cytoplasmic tyrosine phosphotase) and matrix metalloproteinase-1 (MMP-1) in the migration of mouse embryo fibroblast cell. Methods: Shp-2 -/-embryo fibroblast was separated from Shp-2 knockout mouse on E10. 5, and Shp-2 cDNA was transfected into Shp-2 -/- cell. The cell migration was observed with the wound healing, the MMP-1 expression and secretion was analyzed by immunoblotting and immunoprecipitation. The activation of MMP-1 in the supatant was detected with type Ⅰ collagenase activity assay after the stimulation of IL-1α compared with E10. 5 mouse embryo fibroblast cell(Shp-2+/+). Results: There was obvious migration in Shp-2+/+ cells and Shp-2-/-R cells, but not in Shp-2 -/- cells. The shape of Shp-2 -/- cell was epithelial-like. The expression and secretion was increased in Shp-2+/+cells and Shp-2-/-Rcells, and it had not changed in Shp-2 -/- cell. The activation of MMP-1 was lower in Shp-2-/-cells compared to the other cells. Conclusion: IL-1 induces the expression and secretion of MMP-1α at the physical dose, and the cell migration is involved in MMP-1 by way of Shp-2 signal transduction.