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目的了解2012年广西一起成人流行性腮腺炎(流腮)暴发的病原基因型特征,为制定防治流腮策略提供依据。方法将病例咽拭子接种到淋巴信号激活因子转染的非洲绿猴肾细胞(Vero/SLAM)进行病毒分离,应用逆转录-聚合酶链反应(RT-PCR)扩增小疏水蛋白(SH)基因片段,进行测序和分析。结果从8个病例咽拭子标本中,分离到4株腮腺炎病毒,均属于F基因型,与国内外近年流行的其他F基因型腮腺炎病毒株核苷酸和氨基酸同源性分别为93.9%-99.6%和91.2%-100%,其中与2009年广西株核苷酸同源性和氨基酸同源性分别为95.8%-96.5%和92.9%;与中国疫苗株S79核苷酸差异高达14.3%。结论该疫情是由F基因型腮腺炎病毒引起的,与2009年广西株属于两个不同的传播链。应加强流腮的实验室监测,以及时有效地预防和控制流腮暴发。
Objective To understand the characteristics of the pathogen genotypes in 2012 with the outbreak of adult mumps (MMR) in Guangxi and provide evidence for the development of strategies for prevention and treatment of mumps. Methods Throat swabs were inoculated into Vero / SLAM cells transfected with lymphotactin, and then the small hydrophobic protein (SH) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) Gene fragments, sequenced and analyzed. Results Four mumps viruses were isolated from throat swab samples of eight cases and belong to genotype F. The homology of the nucleotide and amino acid with other F genotype mumps strains in recent years was 93.9 % -99.6% and 91.2% -100%, respectively. Among them, nucleotide homology and amino acid homology of Guangxi strain were 95.8% -96.5% and 92.9% respectively in 2009 and 14.3% respectively with Chinese vaccine strain S79 %. Conclusion The outbreak was caused by the F genotype mumps virus, which belonged to two different transmission chains in 2009. Laboratory monitoring of gills should be strengthened in order to prevent and control the occurrence of gills in time and effectively.