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以强休眠玉米自交系08-641为试验材料,分别对处于休眠状态下的新鲜收获种子和经过10 d后熟作用破除休眠的种子进行了蛋白质组学差异表达分析。结果表明,通过双向电泳技术在3次重复试验下休眠状态的08-641鲜种子蛋白2-DE图谱上共检测到约600个蛋白质点,在经过10 d后熟作用破除休眠的08-641种子蛋白2-DE图谱上共检测到约620个蛋白质点,其中下调表达蛋白质点4个,上调表达蛋白质点4个,新增蛋白质点8个,缺失表达蛋白质点7个。经过质谱鉴定的差异表达蛋白质主要涉及球蛋白、胚胎晚期丰富蛋白、豆球蛋白等贮藏物蛋白质;蛋白酶体、山梨醇脱氢酶等参与物质代谢的蛋白质;热激蛋白等参与蛋白质结构、细胞功能调控的蛋白质。推测08-641种子休眠是由于种子内休眠相关蛋白的过量表达或缺失抑制了种子的正常萌发。
Using the strongly dormant maize inbred line 08-641 as the experimental material, the differentially expressed proteomic analysis of freshly harvested seeds and dormant seeds after 10 days of ripening were performed respectively. The results showed that about 600 protein spots were detected on the 08-641 fresh seed protein 2-DE map of the dormant state by three-fold electrophoresis in two-way electrophoresis technique, and the dormant 08-641 seed was destroyed after 10 days of ripening A total of about 620 protein spots were detected on protein 2-DE map, including 4 down-regulated protein spots, 4 up-regulated protein spots, 8 new protein spots and 7 missing protein spots. Differentially expressed proteins identified by mass spectrometry mainly involve proteins in storage proteins such as globin, late embryonic abundance protein and soya bean protein; proteins involved in substance metabolism such as proteasome and sorbitol dehydrogenase; and heat shock proteins are involved in protein structure and cell function Regulated proteins. It is speculated that dormancy of 08-641 seeds is due to the overexpression or absence of dormant-related proteins in seeds, which inhibits the normal seed germination.