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实验用雄性SD大鼠,体重250～350g,随机分为四组:假缺血+安慰剂(12只);假缺血+Iloprost(12只);缺血+安慰剂(12只)和缺血+Iloprost(12只).乙醚麻醉,气管插管并作左侧V插管给药.经第四肋间开胸,挤出心脏,结扎冠脉左侧前降支.冠脉左侧前降支的第一个主要分支为室间隔动脉,该动脉不结扎,以室间隔为非缺血区(NMI)作对照.结扎后将心脏放回并缝合关闭胸腔.6h后,处死动物,取出心脏,放入冰冷的0.9%NaCl溶液中,并作肌酸激酶(CK)活性的测定;用一维薄层层析法测定总磷脂含量;用二维法测定个别磷脂含量.实验发现,前列腺环素(PGI_2)的稳定类似物Iloprost能明显提高缺血区心肌的CK活性.在假缺
Male Sprague-Dawley rats weighing 250-350 g were randomly divided into four groups: fake ischemia + placebo (12 rats); false-ischemic + Iloprost (12 rats); ischemia + placebo Blood + Iloprost (12) .Aether anesthesia, endotracheal intubation and as the left V intubation through the fourth intercostal thoracotomy, out of the heart, ligation of the left anterior descending coronary artery The first major branch of the descending branch was the septal artery, which was non-ligated and interventricular septum was used as a control in the non-ischemic area (NMI) .After ligation, the heart was placed back and the suture was closed to close the thorax.6h later, the animals were sacrificed and removed Heart, placed in ice-cold 0.9% NaCl solution, and creatine kinase (CK) activity determination; one-dimensional thin layer chromatography determination of total phospholipid content; two-dimensional determination of individual phospholipid content experiments found that the prostate Iloprost, a stable analogue of cyclooxygenase (PGI_2), significantly increased CK activity in the ischemic myocardium.