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反义寡核苷酸可以特异性地抑制基因表达，将ＨＬＡ－ＤＰＢ＿１特异性反义寡核苷酸加入细胞培养基中，对ＨＬ－６０和Ｋ５６２白血病细胞株由γ－干扰素诱导的ＨＬＡ－ＤＰＢ＿１表达有特异性抑制作用。当反义寡核苷酸浓度在４０μｇ／ｍｌ时，１小时后即出现ＨＬＡ－ＤＰＢ＿１基因表达抑制现象，持续时间达４８小时，７２小时后表达又逐渐恢复；反义寡核苷酸抑制基因表达有很高的特异性，在ＨＬＡ－ＤＰＢ＿１表达完全被封闭的情况下，与它链锁紧密的ＤＱＢ＿１、ＤＲＢ＿１表达依然如常。另外，不同浓度的反义寡核苷酸，从５μｇ／ｍｌ到６０μｇ／ｍｌ，对两种细胞株￣３Ｈ－ＴｄＲ掺入无明显影响，说明它对细胞生长影响不大。
Antisense oligonucleotide can specifically inhibit gene expression, the HLA-DPB 1 specific antisense oligonucleotide added to the cell culture medium, HL-60 and K562 leukemia cell line induced by γ-interferon HLA- DPB_1 expression specific inhibition. When the antisense oligonucleotide concentration was 40μg / ml, the inhibition of HLA-DPB_1 gene expression appeared after 1 hour, lasting for 48 hours, and then gradually recovered after 72 hours. The antisense oligonucleotide inhibited the gene expression It has high specificity. When the expression of HLA-DPB_1 is completely blocked, its chain-locked DQB_1 and DRB_1 expression are still normal. In addition, different concentrations of antisense oligonucleotide from 5μg / ml to 60μg / ml had no significant effect on 3H-TdR incorporation in both cell lines, indicating that it had little effect on cell growth.