论文部分内容阅读
目的研究大鼠脑梗死灶周围巨噬细胞游走抑制因子(MIF)的表达及变化,探讨MIF在脑梗死过程中的作用及意义。方法采用电凝法构建大鼠大脑中动脉闭塞模型,随机分为假手术组8只和脑梗死组48只,其中脑梗死组又分为4 h、12 h,1 d、3 d,1周、2周6个时间点,每个时间点8只,应用蛋白免疫印迹和荧光定量PCR技术检测脑梗死大鼠梗死灶周围MIF蛋白含量及其mRNA的表达水平。结果脑梗死组大鼠梗死灶周围MIF蛋白及mRNA表达在梗死后逐渐升高,梗死后4 h、12 h,1 d、3 d明显高于对照组(P<0.05)。大鼠梗死灶MIF蛋白含量与mNSS评分和MIF mRNA呈正相关(r=0.661,P=0.000;r=0.733,P=0.000)。结论大鼠脑梗死灶周围MIF蛋白及其mRNA表达水平呈先增高后恢复正常的变化规律,提示MIF在脑梗死病变过程中发挥重要作用。
Objective To study the expression and changes of macrophage migration inhibitory factor (MIF) in rat cerebral infarction and to explore the role and significance of MIF in cerebral infarction. Methods The middle cerebral artery occlusion model was established by electrocoagulation and divided into sham operation group (n = 8) and cerebral infarction group (n = 48). The cerebral infarction group was further divided into 4 h, 12 h, 1 d, 3 d, , Two weeks and six time points, each time point of 8, the application of Western blot and fluorescence quantitative PCR technology to detect cerebral infarction around MIF protein content and mRNA expression levels. Results The expression of MIF protein and mRNA in the infarct group increased gradually after infarction in infarction group. The infarct size was significantly higher at 4 h, 12 h, 1 d and 3 d after infarction (P <0.05). There was a positive correlation between MIF protein level and MNSS score and MIF mRNA level in infarct area of rats (r = 0.661, P = 0.000; r = 0.733, P = 0.000). Conclusion The expression of MIF protein and its mRNA in cerebral infarct focus first increased and then returned to normal, indicating that MIF plays an important role in the pathogenesis of cerebral infarction.