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目的观察在离心机高Ｇ值反复作用下，血脑屏障通透性的变化特点，为高Ｇ值作用下脑功能改变的解释提供生理学依据。方法选雄性大鼠２０只，分为对照组及＋ＧＺ暴露后不同时间组，实验组经增长率为１Ｇ／ｓ，持续３ｍｉｎ的＋１０ＧＺ暴露３次，立即经大鼠心脏灌注镧固定液，开颅取脑，制成切片，置透射电镜下观察。结果＋ＧＺ重复暴露３次后即刻，即见镧颗粒通过少数开放的毛细血管内皮细胞间的紧密连接，沉积于血管外围；暴露后１ｈ及６ｈ，可见大部分毛细血管内皮细胞间的紧密连接开放，镧颗粒弥漫性地分布于神经细胞间隙，部分镧颗粒已进入神经细胞内部，分布于线粒体、内质网等细胞器的表面；暴露后２４ｈ，则见镧颗粒仅局限分布在血管腔内，毛细血管外的间隙则未见镧颗粒沉积，与对照组大鼠相似。结论＋１０ＧＺ／３ｍｉｎ重复作用３次可引起大鼠血脑屏障通透性增加，这种改变是可逆的。其可能在＋ＧＺ致脑水肿的发生、发展过程中起重要作用。
Objective To observe the changes of the permeability of the blood-brain barrier under the repeated high G value of centrifuge and to provide the physiological basis for the explanation of the changes of brain function under the high G value. Methods Twenty male rats were randomly divided into control group and different time groups after + GZ exposure. The experimental group was exposed to + 10GZ for 3min with a growth rate of 1G / s. The rats were immediately perfused with lanthanum fixing solution Take the brain, made into slices, placed under transmission electron microscope observation. Results + GZ repeated exposure three times immediately, that is, lanthanum particles through a small number of open capillary endothelial cell adhesion between the deposition in the vascular periphery; exposed 1h and 6h, showing most of the capillary endothelial cell tight junction open, Lanthanum particles diffusely distributed in the nerve cell gap, some lanthanum particles have entered the nerve cells, distributed in mitochondria, endoplasmic reticulum and other organelles surface; 24h after exposure, then see lanthanum particles are localized in the vascular cavity, capillary Lack of lanthanum particles was observed outside the gap, similar to the control rats. Conclusion Repeated + 10GZ / 3min 3 times can cause the increase of BBB permeability in rats, which is reversible. It may play an important role in the development of brain edema induced by + GZ.