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目的观察微小RNA(mi R)-383对骨肉瘤细胞生物学特性的影响。方法脂质体转染骨肉瘤细胞(U-2OS),检测对细胞凋亡、增殖的影响,并检测细胞迁移侵袭能力、克隆形成能力的改变。结果细胞凋亡情况:空白对照组、无义序列组和实验组细胞的凋亡率分别为(8.56±1.45)%、(12.6l±1.37)%、(33.20±2.59)%,差异有统计学意义(P<0.05);细胞迁移实验:空白对照组、无义序列组和实验组分别为(82±5)个、(68±3)个和(45±7)个,差异有统计学意义(P<0.05);细胞迁移与侵袭实验:空白对照组、无义序列组和实验组细胞穿膜数分别为(82±5)个、(68±3)个和(45±7)个,以及(35±9)个、(32±4)个和(13±5)个,差异有统计学意义(P<0.05);克隆形成实验:空白对照组、无义序列组和实验组克隆数分别为(12.7±4.1)个、(12.3±3.6)个和(6.4±2.7)个。结论抑制mi R-383使骨肉瘤细胞凋亡增加,使细胞增殖、侵袭与迁移及克隆形成受到抑制。
Objective To observe the effect of microRNA (mi R) -383 on the biological characteristics of osteosarcoma cells. Methods The liposomal transfection of osteosarcoma cells (U-2OS) was performed to detect the effects on cell apoptosis and proliferation. The cell migration, invasiveness and clonogenic capacity were also examined. Results Apoptosis: The apoptotic rates in control group, non-sense sequence group and experimental group were (8.56 ± 1.45)%, (12.61 ± 1.37)%, (33.20 ± 2.59)%, respectively, with statistical difference (82 ± 5), (68 ± 3) and (45 ± 7), respectively, with significant difference (P <0.05). In the cell migration experiment, (82 ± 5), (68 ± 3) and (45 ± 7), respectively, in the blank control group, the nonsense sequence group and the experimental group (P <0.05) (35 ± 9), (32 ± 4), and (13 ± 5), respectively. There was significant difference between the two groups (P <0.05). Clone formation assay: Blank control group, nonsense sequence group and experimental group (12.7 ± 4.1), (12.3 ± 3.6) and (6.4 ± 2.7), respectively. Conclusion Inhibition of mi R-383 increases the apoptosis of osteosarcoma cells and inhibits the proliferation, invasion, migration and colony formation.