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近来发现,心脏不仅是血液循环的动力器官,而且还具有内分泌功能。例如,有强大利钠、利尿作用的心钠素(ANF)就主要存在于心房肌细胞的特殊颗粒(SG)内。其分泌过程是否与其他内分泌细胞一样,尚不清楚。本实验采用给小鼠钠负荷,以促进ANF分泌;禁水使ANF分泌减少的方法,用免疫电镜技术观察,探讨心房肌细胞分泌ANF的形态学依据。材料和方法:一、动物分组:实验用昆明种小鼠20只,体重29.1±2.6g,随机分为禁水,钠负荷
Recently found that the heart is not only the driving force of blood circulation, but also has endocrine function. For example, atrial natriuretic factor (ANF), which has a strong natriuretic and diuretic effect, is found mainly in the special granules (SG) of atrial myocytes. Whether its secretion process is the same as that of other endocrine cells is unclear. In this experiment, the sodium load was applied to the mice to promote the secretion of ANF. The method of reducing the secretion of ANF by waterblocking was used to observe the morphological characteristics of ANF secreted by atrial myocytes by immunoelectron microscopy. Materials and methods: One, animal grouping: 20 Kunming mice were used in experiment, weighing 29.1 ± 2.6g, were randomly divided into water forbidden, sodium load