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作者采用黄疸出血群赖型017株钩端螺旋体(钩体)外膜免疫BALB/c小鼠,通过细胞融合等技术,获得单克隆抗体E4B7D5。亚类鉴定为IgG_1,凝集效价为1:25 600。被动免疫保护试验证明:将单克隆抗体E4B7D5腹水稀释100倍,给实验组豚鼠每只每天腹腔注射1ml,连续6天,能使豚鼠在接受同型017株钩体2ml(每毫升1×10~3条)的攻击后,存活率达80％,且存活20天的豚鼠处死解剖,未见任何病变;3个对照组的存活率分别为10％,20％和10％,该三组仅有4只存活20天,经处死解剖,其中 3只发现较多的陈旧性肺出血。作者认为,单克隆抗体E4B7D5对同型017株钩体有免疫保护作用。
In this study, BALB / c mice were immunized with 017 strains of Leptospira interrogans (Leptospira) of the jaundice hemorrhagic group, and the monoclonal antibody E4B7D5 was obtained by the technique of cell fusion. The subclass was identified as IgG_1 and the agglutination titer was 1:25 600. Passive immune protection test proved that the monoclonal antibody E4B7D5 ascites diluted 100 times per day to the experimental group of guinea pigs intraperitoneal injection of 1ml for 6 days, can make guinea pigs in the same type 017 hook 2ml (1 x 10 ~ 3 ), The survival rate was 80%, and the guinea pigs that survived for 20 days were killed and dissected without any pathological changes. The survival rates of the three control groups were 10%, 20% and 10% Survived only 20 days after sacrifice anatomy, of which 3 found more old pulmonary hemorrhage. The authors believe that the monoclonal antibody E4B7D5 has the same immunopotentiating effect on 017 leptospira.