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The molecular weight separation and column dispersion for a number of SEC columns packedwith various packing materials were calibrated simultaneously using narrow MWD polystyrene stan-dards as probe. The molecular weight dependence of the spreading factor is closely related to thesurface structure in the pore of packings.
The molecular weight separation and column dispersion for a number of SEC columns packed with various packing materials were calibrated simultaneously using narrow MWD polystyrene stan-dards as probe. The molecular weight dependence of the spreading factor is closely related to the surface structure in the pore of packings.