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在培养的鸡胚心室肌细胞上，我们采用膜片钳技术的细胞贴附式构型，研究了黄连素（Ｂｅｒｂｅｒｉｎｅ，ＢＲ）对细胞膜钙单通道电流的影响。结果表明：ＢＲ可使心室肌细胞膜钙单通道电流明显增加；钙通道可利用率增加１１４．２％（Ｐ＜０．００１）；钙通道开放概率增加７５．３％（Ｐ＜０．０５）。用心得安阻断β受体或用异搏定阻断钙通道，均可逆转ＢＲ的上述作用。以上提示：①ＢＲ是一种钙通道激活剂；②ＢＲ对钙通道的调节作用依赖于β受体介导的磷酸化过程。
In cultured chick embryo ventricular myocytes, we used the patch-clamp technique to study the effect of Berberine (BR) on the single-channel current in the cell membrane. The results showed that: BR can significantly increase the single-channel calcium current in ventricular myocytes; the availability of calcium channel increased by 114.2% (P <0.001); the open probability of calcium channel increased by 75.3% (P <0.05) . Blockade of β receptors with orbital occlusion of calcium channels with verapamil can reverse the above effects of BR. The above tips: ① BR is a calcium channel activator; ② BR regulation of calcium channels depends on the β receptor-mediated phosphorylation.