论文部分内容阅读
目的:观察肿节风颗粒对小型猪腮腺照射后活性氧簇(reactive oxygen species,ROS)的清除作用。方法:60只小型猪随机分成空白对照组(对照组)、单纯照射组(单照组)和肿节风加照射组(药照组)3组,每组20只;每组平行分成a、b、c和d 4个亚组,每组5只,分别于照射结束后1、10、40和90d取腮腺组织,药照组于照射前1周开始给予肿节风颗粒(0.3g/kg),直至腮腺组织取出,对照组与单照组给予等量生理盐水。在全麻状态下肿节风组跟单照组给予60 C0γ射线双侧腮腺照射,30Gy/(5f.5w),对照组不予照射,观察各组腮腺质量及ROS含量变化。结果:照射后1d,对照组、单照组、药照组ROS含量分别为(62.58±8.96)、(136.75±33.78)和(62.83±35.25)U/mL,对照组与药照组差异无统计学意义,P>0.01;单照组与药照组和对照组之间差异有统计学意义,P<0.01;照射后10d,对照组、单照组、药照组ROS含量分别为(64.50±8.40)、(338.50±30.36)和(281.67±48.72)U/mL,三组之间差异均有统计学意义,P<0.01;照射后40d,对照组、单照组、药照组ROS含量分别为(62.92±6.34)、(392.83±32.66)和(324.42±54.57)U/mL,三组之间差异均有统计学意义,P<0.01;照射后90d,对照组、单照组、药照组ROS含量分别为(62.75±7.01)、(438.92±36.31)和(323.75±49.30)U/mL,三组之间差异均有统计学意义,P<0.01;药照组的ROS含量均低于单照组。结论:肿节风通过清除小型猪腮腺放射后的ROS活性,能有效减缓腮腺放射损伤。
OBJECTIVE: To observe the scavenging effect of Zengjiefo granules on reactive oxygen species (ROS) in parotid gland of miniature pigs. Methods: Sixty miniature pigs were randomly divided into blank control group (control group), simple irradiation group (single control group) and treatment group plus irradiation group (control group), with 20 rats in each group. Each group was divided into a, b, c and d 4 subgroups, 5 rats in each group. The parotid glands were taken 1, 10, 40 and 90 days after the irradiation respectively. The mice in the control group were treated with Dayangfeng granules (0.3g / kg ) Until the parotid gland tissue removed, the control group and single control group given the same amount of saline. Under general anesthesia, the swollen-air-stroke group was given 60 C0γ-ray bilateral parotid gland irradiation with 30 Gy / (5f. 5w), while the control group was not irradiated. The changes of parotid gland quality and ROS content in each group were observed. Results: At 1 day after irradiation, ROS levels in control group, monotherapy group and drug group were (62.58 ± 8.96), (136.75 ± 33.78) and (62.83 ± 35.25) U / mL, respectively The difference was statistically significant (P <0.01). The levels of ROS in control group, monotherapy group and drug group were (64.50 ± 8.40), (338.50 ± 30.36) and (281.67 ± 48.72) U / mL respectively. The differences among the three groups were statistically significant (P <0.01) (62.92 ± 6.34), (392.83 ± 32.66) and (324.42 ± 54.57) U / mL, the difference between the three groups was statistically significant, P <0.01; 90 d after irradiation, the control group, The levels of ROS were (62.75 ± 7.01), (438.92 ± 36.31) and (323.75 ± 49.30) U / mL, respectively, with significant difference between the three groups (P <0.01) Single photo group. CONCLUSION: The swollen-section wind can effectively reduce the parotid radiation injury by clearing the ROS activity of the parotid gland after radiotherapy.