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目的:检测海藻硫酸甘油糖脂化合物(SQDG)的体外抗肿瘤活性,为寻找有效的抗癌新药提供理论依据。方法:采用MTT法检测SQDG对正常细胞的细胞毒性。在倒置显微镜下观察7种人类肿瘤细胞经SQDG处理72 h后的形态学变化,并用MTT法定量测定其生长抑制情况,同时应用流式细胞术检测细胞周期的改变。结果:SQDG对正常细胞Vero和MC的毒性都很小,其CC50值均大于500μg/mL。它对人乳腺癌细胞MCF-7的生长抑制效果最明显,IC50值为69.1μg/mL,在质量浓度为125μg/mL时完全抑制MCF-7细胞生长。SQDG对H460、A549、HEp2和HeLa细胞也有一定的抑制效果,但对HepG2和A375细胞几乎没有影响。流式细胞术检测发现,SQDG能扰乱MCF-7的细胞周期,促使细胞凋亡或坏死,从而产生有效的生长抑制效果。但SQDG对H460和HepG2细胞周期的改变无统计学意义。结论:硫酸甘油糖脂SQDG对正常细胞无毒害,能选择性地有效抑制体外培养的乳腺癌细胞的生长,是一种有发展潜力的抗肿瘤药物。
Objective: To detect in vitro antitumor activity of seaweed glyceryl sulfate compound (SQDG), providing a theoretical basis for finding effective anti-cancer drugs. Methods: The cytotoxicity of SQDG to normal cells was detected by MTT assay. The morphological changes of 7 kinds of human tumor cells treated with SQDG for 72 h were observed under an inverted microscope. The growth inhibition was quantitatively determined by MTT assay. The cell cycle was also detected by flow cytometry. Results: The toxicity of SQDG to normal cells Vero and MC was very small, and the CC50 value of SQDG was more than 500μg / mL. It has the most obvious inhibitory effect on the growth of human breast cancer cells MCF-7 with an IC50 value of 69.1μg / mL and completely inhibits the growth of MCF-7 cells at a concentration of 125μg / mL. SQDG also had some inhibitory effects on H460, A549, HEp2 and HeLa cells but had little effect on HepG2 and A375 cells. Flow cytometry found that SQDG can disrupt the cell cycle of MCF-7, promote apoptosis or necrosis, resulting in effective growth inhibitory effect. But SQDG had no significant change on the cell cycle of H460 and HepG2. CONCLUSIONS: Soy-glycero-glycolipid SQDG is non-toxic to normal cells and can selectively inhibit the growth of breast cancer cells cultured in vitro. It is an antitumor drug with potential for development.