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目的 :探讨人巨细胞病毒 (CMV)在动脉粥样硬化发生和发展中的作用。 方法 :采用流式细胞计数仪观察了人 CMV对离体培养的人脐静脉内皮细胞 (HUVEC)增殖和凋亡的影响。本实验分为 5组 ,分别为正常对照组 ,肿瘤坏死因子细胞凋亡组 ,无血清培养细胞凋亡组 ,CMV感染 +肿瘤坏死因子细胞凋亡组 ,CMV感染 +无血清培养细胞凋亡组。 结果 :实验发现无血清培养和肿瘤坏死因子诱导的细胞凋亡率较正常对照组升高 ,而 CMV感染的细胞凋亡率明显降低 ;细胞计数的结果表明 CMV感染后 3天的 HUVEC细胞数是未感染的 5倍。流式细胞仪的检测结果表明 :CMV感染细胞的增殖指数明显高于正常对照组 ,CMV可使细胞耐受无血清培养条件 ,保持细胞增殖。 结论 :人 CMV感染 HUVEC后能够改变 HUVEC的细胞增殖过程 ,抑制细胞凋亡 ,HUVEC的过度增生及 HUVEC感染引起的炎性反应可能影响内皮细胞的功能 ,甚至破坏内皮细胞 ,导致血栓形成、脂质代谢紊乱 ,最终形成动脉粥样硬化。
Objective: To investigate the role of human cytomegalovirus (CMV) in the development of atherosclerosis. Methods: The effect of human CMV on the proliferation and apoptosis of cultured human umbilical vein endothelial cells (HUVECs) was observed by flow cytometry. The experiment was divided into five groups, namely normal control group, tumor necrosis factor apoptosis group, serum-free cultured apoptosis group, CMV infection + tumor necrosis factor apoptosis group, CMV infection + serum-free cultured apoptosis group . Results: The results showed that the apoptosis rate induced by serum-free culture and tumor necrosis factor (TNF) -mediated apoptosis was higher than that of the normal control group, while the apoptosis rate of CMV-infected cells was significantly decreased. The cell count showed that the number of HUVEC cells Uninfected 5 times. Flow cytometry results showed that: CMV infected cells proliferation index was significantly higher than the normal control group, CMV can make the cells resistant to serum-free culture conditions, to maintain cell proliferation. CONCLUSION: HUVEC infected with human CMV can change the cell proliferation process and inhibit the apoptosis of HUVEC. The hyperproliferation of HUVEC and the inflammatory reaction induced by HUVEC infection may affect the function of endothelial cells and even destroy the endothelial cells, resulting in thrombosis, Metabolic disorders eventually lead to atherosclerosis.