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目的:合成一种由可活化细胞穿膜肽ACPP、水溶性高分子聚合物pHPMA修饰腺病毒的接合物,并初步探索该接合物逃逸腺病毒中和抗体免疫清除的能力。方法:通过化学合成的方法合成高分子聚合物pHPMA及可活化细胞穿膜肽ACPP,通过化学合成的方法合成了接合物ACPP-pc-Ad.egfp。通过动态光散射检测高分子聚合物ACPP-pc-Ad.egfp的粒径,通过酶标仪检测接合物ACPP-pc-Ad.egfp感染能力,通过酶标仪检测接合物ACPP-pc-Ad.egfp感染能力免疫逃逸能力。结果:成功合成了高分子聚合物pHPMA及可活化细胞穿膜肽ACPP;动态光散射检测结果表明ACPP及pHPMA修饰的腺病毒接合物粒径增大;酶标仪检测接合物ACPP-pc-Ad.egfp具有较强的感染A549细胞的能力,并对腺病毒中和抗体的免疫清除发生了明显的免疫逃逸。结论:成功合成了ACPP-pc-Ad.egfp接合物,并证实ACPP-pc-Ad.egfp能对机体的腺病毒免疫清除发生免疫逃逸。
OBJECTIVE: To synthesize an adenovirus conjugate modified by activated cell penetrating peptide ACPP and water-soluble polymer pHPMA, and to explore the ability of the conjugate to escape the immune clearance of adenovirus neutralizing antibody. Methods: The polymer PPP-pc-Ad.egfp was synthesized by chemical synthesis method. The conjugate ACPP-pc-Ad.egfp was synthesized by chemical synthesis. The particle size of the polymer ACPP-pc-Ad.egfp was detected by dynamic light scattering. The ACPP-pc-Ad.egfp conjugate was detected by microplate reader. The conjugate ACPP-pc-Ad was detected by microplate reader. egfp infection ability immune escape ability. RESULTS: The high-molecular weight polymer (pHPMA) and ACPP (Activated Cell-Penetrating Peptide) were successfully synthesized. The results of dynamic light scattering showed that the particle size of ACPP and pHPMA modified adenovirus conjugates increased. The conjugate ACPP-pc-Ad .egfp has a strong ability to infect A549 cells, and a clear immune escape occurred on the immune clearance of adenovirus neutralizing antibodies. Conclusion: ACPP-pc-Ad.egfp conjugate was successfully synthesized and confirmed that ACPP-pc-Ad.egfp could escape immune evasion of adenovirus.