论文部分内容阅读
1983年9月,从新疆石河子豇豆病毒病植株上分离到1株病毒分离物Cp-1。汁液摩擦接种试验的结果证明,它可以侵染3种豆科植物和2种藜科植物。在豇豆上引起系统花叶,叶片皱缩下卷,并有疱疹等症状;在绿豆上表现为局部棕褐色环纹;在苋色藜、昆诺阿藜上引起局部病斑。体外抗性测定,失毒温度为55—60℃,稀释限点为10~(-3)—10~(-4)。体外保毒期3—4天。Cp-1易汁液摩擦接种,桃蚜、棉黑蚜均可传毒,可通过种子传毒,种传率为10.6％。病毒粒体线条状,大小为12—15×700—750毫微米。病株叶片细胞内有风轮状及环状内含体。该分离物与豇豆蚜传花叶病毒(CABMV)的抗血清形成明显的沉淀线。根据以上性状,分离物Cp-1属于马铃薯Y病毒组的豇豆蚜传花叶病毒。在新疆各地采取21个标样,经血清学和寄主范围测定,有13个标样属该病毒,约占62％。说明豇豆蚜传花叶病毒在新疆种植的豇豆上是普遍存在的。
In 1983 September, from Xinjiang Shihezi cowpea virus disease isolates a virus isolate Cp-1. The results of the juice friction inoculation test proved that it can infect 3 leguminous plants and 2 Chenopodiaceae plants. On the cowpea caused by the system of mosaic, leaf folds down the volume, and herpes and other symptoms; in the mung bean showed a local brown ring; in amaranth, quinoa caused by local lesions. In vitro resistance assay, the temperature of detoxification was 55-60 ℃, and the dilution limit was 10 -3 -3 -10 -4. In vitro protection of 3-4 days. C-1 easy juice friction inoculation, Myzus persicae, cotton black aphids can be poisoned, can be poisoned by seeds, seed rate was 10.6%. Viral mitochondria, the size of 12-15 × 700-750 nm. The diseased leaves have round and annular inclusions in the cells. The isolate formed a clear precipitation line with the antisera of cowpea aphid-transmitted mosaic virus (CABMV). According to the above traits, isolate Cp-1 belongs to the cowpea aphid-transmitted mosaic virus of the potato Y virus group. Twenty-one standard samples were taken from all over Xinjiang, and the serotypes and host range were determined. Thirteen standard samples belonged to the virus, accounting for about 62%. That cowpea aphid-transmitted mosaic virus is planted in Xinjiang cowpea ubiquitous.