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AIM:This study investigated the anti-cancer effect ofcombined quercetin and a recombinant adenovirus vectorexpressing the human p53,GM-CSF and B7-1 genes(designated BB-102)on human hepatocellular carcinoma(HCC)cell lines in vitro.METHODS:The sensitivity of HCC cells to anticancer agentswas evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.The viability of cells infectedwith BB-102 was determined by trypan blue exclusion.Theexpression levels of human wild-type p53,GM-CSF and B7-1genes were determined by Western blot,enzyme-linkedimmunosorbent assay(ELISA)and flow cytometric analysis,respectively.The apoptosis of BB-102-infected or quercetin-treated HCC cells was detected by terminal deoxynucleotidyltransferase(TdT)assay or DNA ladder electrophoresis.RESULTS:Quercetin was found to suppress proliferation ofhuman HCC cell lines BEL-7402,HUH-7 and HLE,with peaksuppression at 50μmol/L quercetin.BB-102 infection wasalso found to significantly suppress proliferation of HCC celllines.The apoptosis of BB-102-infected HCC cells was greaterin HLE and HUH-7 cells than in BEL-7402 cells.Quercetin didnot affect the expression of the three exogenous genes inBB-102-infected HCC cells(P>0.05),but it was found to furtherdecrease proliferation and promote apoptosis of BB-102-infected HCC cells.CONCLUSION:BB-102 and quercetin synergeticallysuppress HCC cell proliferation and induce HCC cell apoptosis,suggesting a possible use as a combined anti-cancer agent.
AIM: This study investigated the anti-cancer effect of combined quercetin and a recombinant adenovirus vectorexpressing the human p53, GM-CSF and B7-1 genes (designated BB-102) on human hepatocellular carcinoma (HCC) cell lines in vitro. METHODS: The sensitivity of HCC cells to anticancer agentswas evaluated by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay.The viability of cells infected with BB-102 was determined by trypan blue exclusion. levels of human wild-type p53, GM-CSF and B7-1genes were determined by Western blot, enzyme-linked immunosorbent assay (ELISA) and flow cytometric analysis, respectively. The apoptosis of BB-102-infected or quercetin-treated HCC cells was detected by terminal deoxynucleotidyltransferase (TdT) assay or DNA ladder electrophoresis .RESULTS: Quercetin was found to suppress proliferation of human HCC cell lines BEL-7402, HUH-7 and HLE, with peaksuppression at 50 μmol / L quercetin. B-102 infection was found significantly suppress prolif eration of HCC celllines. The apoptosis of BB-102-infected HCC cells was greater in HLE and HUH-7 cells than in BEL-7402 cells. Quercetin didnot affect the expression of the three exogenous genes in BB- 102-infected HCC cells (P> 0.05), but it was found to further promote proliferation and promote apoptosis of BB-102-infected HCC cells. CONCLUSION: BB-102 and quercetin synergetically supppply HCC cell proliferation and induce HCC cell apoptosis, suggesting a possible use as a combined anti-cancer agent .