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目的:研究柯萨奇B3病毒(CVB3)感染乳鼠心肌细胞缝隙连接蛋白43(CX43)、内质网应激伴侣蛋白GRP94的表达。方法:实验分为对照组(正常细胞)和CVB3感染组(正常细胞+CVB3)。采用免疫组织化学方法检测培养乳鼠心肌细胞α-SMA蛋白,采用real time PCR技术检测各组心肌细胞CX43、GRP94表达。结果:与对照组比较,CVB3感染组心肌细胞GRP94表达增多,CX43表达减少(均P<0.01)。结论:CVB3可引发心肌细胞发生内质网应激,并引起CX43表达减少。
Objective: To investigate the expression of connexin 43 (CX43) and endoplasmic reticulum stress protein GRP94 in neonatal rat cardiomyocytes infected with Coxsackievirus B3 (CVB3). Methods: The experiment was divided into control group (normal cells) and CVB3 infection group (normal cells + CVB3). The protein of α-SMA was detected by immunohistochemical method in neonatal rat cardiomyocytes, and the expression of CX43 and GRP94 in each group were detected by real time PCR. Results: Compared with the control group, the expression of GRP94 and the expression of CX43 decreased in CVB3 infected group (all P <0.01). Conclusion: CVB3 can induce cardiomyocyte endoplasmic reticulum stress and cause a decrease of CX43 expression.