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进一步研究表明:未经干燥含水量38.3％的坡垒种子,其胚根尖细胞的超微结构良好,膜系统完整,各种细胞器能清楚辨认。经不同干燥处理后,含水量分别下降至30.7％、27.1％、22.7%、19.7％时,超微结构发生相应的不同程度变化和破损。与电镜观察试样相对应的胚轴—胚根切段培养试验中,其恢复生长和受害情况与发芽试验结果基本相同。在各含水量下,生长健壮或有生长表现的切段分别为100％、100％、70％、10％、0％。可以看出失水劣变中种子活力降低直至完全丧失发芽力,与细胞超微结构的变化和破损程度紧密相关。液泡膜或核膜的破裂可能是产生不可逆变化的临界点。
Further studies showed that: without the dry water content of 38.3% of the slope seedlings, the root tip cells ultrastructure is good, the membrane system is complete, a variety of organelles can be clearly identified. After different drying treatments, the ultrastructural changes and damage of the ultrastructure were observed when the water content decreased to 30.7%, 27.1%, 22.7% and 19.7% respectively. In the hypocotyl-radicle section culture experiment corresponding to the electron microscope observation sample, the recovery growth and the damage situation are basically the same as the germination test results. At each water content, cuttings with robust or vegetative growth were 100%, 100%, 70%, 10% and 0%, respectively. It can be seen that the seed vigor decreased until the total loss of germination, which is closely related to the changes of cell ultrastructure and the degree of damage. The rupture of the tonoplast or nuclear membrane may be a critical point for irreversible changes.