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AIM:To introduce an animal model of hepatocellularcarcinoma(HCC)in ACI-rats,and to evaluate the therapeuticeffects of Poly-lactide-co-glycolide(PIcg)-microspheres in thetransarterial chemoembolization(TACE)in this model,aswell the value of this model in the experiments ofinterventional therapy.METHODS:Subcapsular implantation of a solid MorrisHepatoma 3 924A(1 mm~3)in the livers was carried out in11 male ACI-rats.The tumor volume(V1)was measured bymagnetic resonance imaging(MRI)(13 days afterimplantation).After laparotomy and retrograde placementof catheter into the gastroduodenal artery(14 days afterimplantation),the following protocols of interventionaltreatment were performed:(A)mitomycin C+Poly-lactide-co-glycolide(PIcg)-microspheres(n=4);(B)0.9 % NaCl(control group,n=7).13 days after these therapies the changeof the tumor volume(V2)was determined by MRI again.RESULTS:The success rate of tumor implantation reachedto 100%.The mean tumor volume before TACE(V1)were0.082 cm~3 in group A and 0.096 cm~3 in group B respectively.The mean tumor volume after TACE(V2)were 0.230 cm~3 ingroup A and 1.347 cm~3 in group B respectively.The meanV2/V1 were 2.860 in group A and 27.120 in group Brespectively.Compared to the control group(group B),groups A showed a significant reduction of tumor growth(P=0.004)in the period of observation.CONCLUSION:The growth of liver tumor could be obviouslyprevented by utilizing Plcg-mitomycin-microspheres in TACEin animal model.This rat model of HCC is suitable for theexperimental studies of interventional therapy.
AIM: To introduce an animal model of hepatocellular carcinoma (HCC) in ACI-rats, and to evaluate the therapeuticeffects of Poly-lactide-co-glycolide (PIcg) -microspheres in the transarterial chemoembolization (TACE) in this model, aswell the value of this model in the experiments of interventional therapy. METHODS: Subcapsular implantation of a solid MorrisHepatoma 3 924A (1 mm ~ 3) in the livers was carried out in 11 male ACI-rats. The tumor volume (Vl) was measured by magnetic resonance imaging (MRI 13 days afterimplantation. After laparotomy and retrograde placement of the catheter into the gastroduodenal artery (14 days afterimplantation), the following protocols of interventionalaltalt were performed: (A) mitomycin C + poly-lactide- co- glycolide (PIcg) 4 days; (B) 0.9% NaCl (control group, n = 7) .13 days after these therapies the change of the tumor volume (V2) was determined by MRI again.RESULTS: The success rate of the tumor implantation reached 100%. mean tumor volume before TACE (V1) were 0.082 cm ~ 3 in group A and 0.096 cm ~ 3 in group B respectively.The mean tumor volume after TACE (V2) were 0.230 cm ~ 3 ingroup A and 1.347 cm ~ 3 in group B respectively.The meanV2 / V1 were 2.860 in group A and 27.120 in group Brespectively .Compared to the control group (group B), groups A showed a significant reduction of tumor growth (P = 0.004) in the period of observation. CONCLUSION: The growth of liver tumor could beprevented by utilizing Plcg-mitomycin-microspheres in TACEin animal model. This rat model of HCC is suitable for the experimental studies of interventional therapy.