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目的:探讨高血压合并动脉粥样硬化与血管紧张素原的相关性。方法:30只雄性16周龄SHR大鼠随机均分为SHR组和SHR合并AS组,另设15只同龄雄性WKY大鼠作为正常血压对照组即WKY组。SHR合并AS组饲以高脂饲料并辅以大剂量VitD3灌胃建立高血压动脉粥样硬化大鼠模型,SHR组和15只同龄雄性WKY大鼠均饲以标准饲料。各组大鼠分别于0、6、12周时光镜、电镜下评估血管病变,全自动生化分析仪检测血脂水平,并采用ELISA法检测血清AGT、AngII浓度。结果:SHR血清AGT、AngII浓度显著高于WKY大鼠(P<0.05)。存在AS病变的SHR合并AS组,血清AGT、AngII浓度明显高于无AS病变的SHR组,且随着AS病变严重性的增加,血清AGT、AngII浓度亦增加(P<0.05)。结论:抑制AGT的表达可能为高血压患者中AS的防治提供一种新的方法。
Objective: To investigate the correlation between hypertension complicated with atherosclerosis and angiotensinogen. Methods: Thirty male SHR rats of 16 weeks old were randomly divided into SHR group and SHR combined with AS group. Fifteen male WKY rats of the same age were randomly divided into normal control group and WKY group. SHR group and 15 male WKY rats of the same age were fed with standard diet. The rats in SHR combined AS group were fed with high-fat diet supplemented with high-dose VitD3 gavage to establish hypertensive atherosclerosis rat model. The rats in each group were assessed by light microscopy and electron microscopy at 0, 6 and 12 weeks respectively. The levels of serum lipids were measured by automatic biochemical analyzer. The levels of serum AGT and AngII were measured by ELISA. Results: The serum levels of AGT and AngII in SHR were significantly higher than those in WKY rats (P <0.05). The serum levels of AGT and AngII in SHR with AS lesions were significantly higher than those in SHR without AS lesions. Serum AGT and AngII levels also increased with the severity of AS (P <0.05). Conclusion: Inhibition of AGT expression may provide a new method for the prevention and treatment of AS in hypertensive patients.