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目的探讨老年人非酒精性脂肪肝(NAFL)与载脂蛋白E(apoE)基因多态性的关系。方法对浙江省台州市农村536例60岁以上老年人和182例健康对照者采用聚合酶链法检测载脂蛋白E基因多态性和等位基因特异性寡核苷酸探针双色法检测112位和158位密码子双突变点,应用颜色补偿档案及熔解曲线分析。ELISA法检测apoE、apoE4基因表达程度。结果NAFL组和肝脂肪浸润(FIL)组以杂合子E2／3为主,分布频率分别为E2／3>E3／4>E3／3>E2／4>E2／2和E2／3>E3／3>E3／4>E2／4;apoE基因表达水平显著上调(P<0．05)。结论老年人NAFL的发生有一定的基因和家族聚集性,E2／3型是遗传易感基因,并且apoE基因112位点和158位点密码子的搭配及纯或杂合子和脂肪肝的发生及其疾病程度密切相关。
Objective To investigate the relationship between NAFL and apolipoprotein E (apoE) gene polymorphisms in the elderly. Methods A total of 536 elderly people over the age of 60 and 182 healthy controls in rural Taizhou, Zhejiang Province were detected by polymerase chain reaction (PCR) and polymorphism of allele-specific oligonucleotide probes Bit and 158 codon double mutation point, the application of color compensation file and melting curve analysis. ELISA method to detect apoE, apoE4 gene expression level. Results The majority of heterozygotes E2 / 3 were found in the NAFL group and the group of fatty liver infiltration (FIL). The frequency of E2 / 3> E3 / 4> E3 / 3> E2 / 4> E2 / 2 and E2 / 3> 3> E3 / 4> E2 / 4, apoE gene expression was significantly increased (P <0.05). CONCLUSIONS: NAFL is associated with gene and familial aggregation in the elderly. E2 / 3 is a gene predisposing gene. The combination of codons 112 and 158 of the apoE gene and the occurrence of pure or heterozygous and fatty liver and The severity of the disease is closely related.