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目的研究SD雌性大鼠卵巢储备功能的增龄性变化,观察初老雌性大鼠血清中抗苗勒氏管激素(AMH)、脱氢表雄酮(DHEA)、雌二醇(E_2)、睾酮(T)浓度的变化,探讨初老雌性大鼠卵巢储备功能是否与DHEA、T之间存在相关性。方法选取SPF级1月龄、2月龄、5月龄、9月龄健康雌性Sprague-Dawley大鼠各20只,不进行任何干预,采集其血液,应用酶联免疫吸附(ELISA)法检测不同月龄大鼠血清中AMH、DHEA、E_2、T的浓度,观察SD雌性大鼠随着年龄的增长,对其卵巢储备功能及雄激素水平分泌的影响。结果 1不同月龄大鼠血清AMH水平比较:雌鼠血清中AMH水平呈曲线性变化,1~2月龄阶段(相当于幼年期至青春期阶段)雌鼠血清中AMH水平逐渐升高,2月龄以后,随着年龄的增加,血清中AMH水平逐渐下降,9月龄雌鼠血清中AMH水平显著降低,为4个阶段的最低水平。2不同月龄大鼠血清DHEA水平的比较:1月龄与9月龄、2月龄与9月龄、5月龄与9月龄大鼠血清DHEA值差异有统计学意义(均P<0.05)。1、2、5月龄(幼年期至性成熟期)雌鼠血清中DHEA水平无明显变化,但9月龄雌鼠血清中DHEA浓度呈下降趋势。3不同月龄大鼠血清E2水平的比较:1月龄、2月龄、5月龄与9月龄大鼠血清E2浓度之间的差异有统计学意义(F=55.697,P<0.05),9月龄大鼠血清E2浓度明显低于1、2、5月龄大鼠。雌鼠血清中E2水平会随着年龄的增加而有所下降。4不同月龄大鼠血清T水平的比较:9月龄大鼠与其余3组相比差异有统计学意义(F=94.165,P<0.05),9月龄大鼠血清T水平明显下降。雌鼠年龄的增加会使得血清中T浓度下降。结论 1不同月龄雌性SD大鼠卵巢储备功能存在差异,随着大鼠月龄的增加,卵巢储备功能呈现下降趋势,9月龄大鼠卵巢储备功能下降。2与血清E2水平相比,AMH是反映卵巢储备功能的较稳定和敏感的指标。3雌性大鼠月龄的增加会导致其血清中DHEA、T水平下降,表明雄激素水平与卵巢储备功能可能存在相关性。
Objective To investigate the age-related changes of ovarian reserve in female SD rats and to observe the effects of anti-mullerian hormone (AMH), DHEA, E2 and testosterone T) concentrations in ovariectomized rats to investigate whether there is a correlation between ovarian reserve function and DHEA, T in first-aged female rats. Methods Twenty healthy female Sprague-Dawley rats aged 1 month, 2 months, 5 months and 9 months old with SPF grade were selected and their blood samples were collected without any intervention. ELISA was used to detect the difference The serum concentrations of AMH, DHEA, E_2, T in the male rats were observed. The effects of aging on the ovarian reserve function and androgen secretion were observed in SD female rats. Results 1 AMH levels in rats at different months of age: AMH levels in the serum of the female rats showed a curve-like change. AMH levels in the serum from 1 to 2 months of age (corresponding to the period of infancy to adolescence) gradually increased, and in February After the age, with the increase of age, serum AMH levels gradually decreased, 9-month-old female rats serum AMH levels were significantly lower, the minimum level of 4 stages. Serum DHEA levels in rats at different months: The differences of serum DHEA between 1-month and 9-month-old, 2-month-old and 9-month-old, 5-month-old and 9-month old rats were statistically significant (all P <0.05 ). There was no significant change in serum DHEA levels at 1, 2, 5 months (juvenile to sexual maturity), but serum DHEA concentrations in 9-month-old females decreased. Serum E2 levels of rats at different months: There was a significant difference of serum E2 between 1 month, 2 months, 5 months and 9 months old rats (F = 55.697, P <0.05) Serum E2 concentrations in 9-month-old rats were significantly lower than those in 1, 2 and 5-month-old rats. E2 levels in female rats serum will decline with age. Serum T levels in rats of different ages: The serum levels of T in 9-month-old rats were significantly lower than those in the other three groups (F = 94.165, P <0.05). Increase in female mice will make the serum T concentration decreased. Conclusion 1 The ovarian reserve function of SD rats with different age is different. With the increase of the age of the rats, the reserve function of the ovary shows a declining trend, while the ovarian reserve function of the 9-month-old rats declines. Compared with serum E2 level, AMH is a more stable and sensitive indicator of ovarian reserve function. 3 month-old female rats increased the level of serum lead to DHEA, T decreased, indicating that androgen levels and ovarian reserve may be related.