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目的:建立人血浆中姜黄素的LC-ESI-MS测定方法。方法:采用Zorbax SB-C18(2.1×100 mm,3.5μm)色谱柱,流动相为甲醇-水溶液(70:30)。电喷雾离子源(ESI),负离子检测,采用选择离子监测方式进行定量分析,检测离子为m/z 367(姜黄素),m/z 479(内标芍药苷)。结果:人血浆中姜黄素浓度在8~2000 ng/ml范围内线性关系良好,最低定量限为8 ng/ml。姜黄素的萃取回收率为81.6%~90.6%,日内及日间RSD都低于10%。结论:该法操作简便、快速、灵敏度高,可用于姜黄素的临床血药浓度分析。
Objective: To establish a method for the determination of curcumin in human plasma by LC-ESI-MS. Methods: Zorbax SB-C18 (2.1 × 100 mm, 3.5 μm) was used as the mobile phase in methanol-water solution (70:30). Electrospray ionization (ESI), negative ion detection, quantitative ion monitoring using selective ion detection mode, the detection ion m / z 367 (curcumin), m / z 479 (internal standard paeoniflorin). Results: The linearity of curcumin in human plasma ranged from 8 ng / mL to 2000 ng / mL. The lowest limit of quantification was 8 ng / ml. Curcumin extraction recovery was 81.6% ~ 90.6%, intraday and daytime RSD less than 10%. Conclusion: The method is simple, rapid and sensitive. It can be used in the analysis of clinical plasma concentration of curcumin.