文心兰OnERS1全长基因克隆及表达分析

• 作 者: 田晓岩 杨翠萍 胡进 闫冰玉 巩笑笑 庄玉粉 潘英文 刘进平
• 机 构: 海南省热带生物资源可持续利用重点实验室海南大学农学院,海南出入境检验检疫局热带植物隔离检疫中心,
• 出 处: 分子植物育种
• 发表日期: 2017年04期
• 关键词: OnERS1 乙烯受体 基因克隆 荧光定量PCR ETR1 基因表达分析 衰老期 生物信息学分析 花器官 唇瓣

为了了解乙烯信号受体在文心兰切花衰老中的作用,利用RACE技术克隆了文心兰乙烯受体基因OnERS1(Gen Bank登录号为KP410729)。生物信息学分析表明,OnERS1基因编码631个氨基酸,分子量为70 773.7,等电点为6.82,是跨膜蛋白,与其它植物的ETR乙烯受体蛋白的相似性在74%~99%之间,属ETR1类乙烯受体。基因表达分析结果表明,OnERS1基因在根和蕊柱中表达量最高,在叶片、侧瓣和花萼中次之,在茎和唇瓣中表达量最低;文心兰切花衰老期间OnERS1基因的表达量在第0~6 d持续增加,第6 d后突然下降,第8 d后下降到一个极低的水平。与此同时,花器官中的乙烯释放从第8 d之前缓慢上升,第8~10 d急剧升高。OnERS1基因的时空表达特异性表达说明它可能与花衰老有关。

In order to understand the role of ethylene signaling receptors in the senescence of cut flowers of Centella asiatica, OnERS1 (Gene Bank Accession No. KP410729) was cloned by RACE technique. Bioinformatics analysis showed that OnERS1 gene encodes 631 amino acids with a molecular weight of 70,773.7 and an isoelectric point of 6.82, which is a transmembrane protein with similarity of 74% -99% with other plant ETR ethylene receptor proteins. Is an ETR1 vinyl receptor. The results of gene expression analysis showed that OnERS1 gene was the highest in roots and pilasters, followed by leaf, lateral lobe and calyx, and had the lowest expression in stem and lip. The expression of OnERS1 In 0 ~ 6 d continued to increase, suddenly dropped after 6 d, after the first 8 d dropped to a very low level. In the meantime, the ethylene release in floral organs increased slowly from the 8th day and rapidly increased from the 8th to the 10th day. The specific expression of OnERS1 gene in space-time indicated that it may be related to the flower senescence.