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目的探讨微小RNA-21(miR-21)对食管癌移植瘤模型肿瘤的作用。方法建立裸鼠食管癌移植瘤模型,分别用miR-21(miR-21组)、miR-21抑制剂(miR-21抑制剂组)和生理盐水进行治疗;应用免疫组化技术检测肿瘤组织中增殖细胞核抗原(Ki-67)、半胱氨酸天冬氨酸特异性蛋白酶(Caspase-3)、锌脂蛋白转录因子(Snail)蛋白的表达。结果成功建立食管癌裸鼠移植瘤模型,miR-21组和miR-21抑制剂组肿瘤大小差异有统计学意义(P<0.05),miR-21组Ki-67阳性表达率[(86.28±14.3)%]和Snail阳性表达率[(88.76±12.35)%]明显高于生理盐水组[(46.72±15.68)%和(72.35±8.36)%]及miR-21抑制剂组[(19.34±10.23)%和(35.26±10.28)%];而miR-21组和生理盐水组的Caspase-3阳性率[(35.27±16.12)%和(45.62±23.32)%]明显低于miR-21抑制剂组[(76.45±18.32)%]。结论 miR-21与食管癌生长和转移相关,抑制miR-21可抑制移植瘤生长和转移,并且通过影响Ki-67、Caspase-3和Snail的表达发挥作用。提示miR-21可作为食管癌临床治疗候选靶基因。
Objective To investigate the effect of microRNA-21 (miR-21) on esophageal carcinoma in vivo. Methods The nude mice model of esophageal carcinoma was established and treated with miR-21 (miR-21 group), miR-21 inhibitor (miR-21 inhibitor group) and normal saline. Immunohistochemistry The expression of Ki-67, Caspase-3 and Snail proteins were detected. Results The tumor model of esophageal carcinoma in nude mice was successfully established. The difference of tumor size between miR-21 group and miR-21 inhibitor group was statistically significant (P <0.05). The positive rate of Ki-67 in miR-21 group was (86.28 ± 14.3 (46.72 ± 15.68)% and (72.35 ± 8.36)%], and miR-21 inhibitor group [(19.34 ± 10.23)%] and Snail positive rate [(88.76 ± 12.35)%] % And (35.26 ± 10.28)%, respectively). The positive rate of Caspase-3 in miR-21 group and saline group was significantly lower than that in miR-21 inhibitor group [(35.27 ± 16.12)% vs (45.62 ± 23.32)%] (76.45 ± 18.32)%]. Conclusions miR-21 is associated with the growth and metastasis of esophageal cancer. Inhibition of miR-21 inhibits the growth and metastasis of xenografts and affects the expression of Ki-67, Caspase-3 and Snail. Tip miR-21 can be used as clinical candidate target gene for esophageal cancer.