交链孢醇单甲醚诱发V_(79)细胞突变的研究

• 作 者: 臧梦维 钱玉珍 刘桂亭 杨红艳 董子明 甄应中 安玉会 苗健 徐友梅
• 机 构: 河南医科大学病理生理教研室,河南医科大学病理生理教研室,河南医科大学病理生理教研室,河南医科大学病理生理教研室,河南医科大学病理生理教研室,河南医科大学食管癌研究室,河南医科大学食管癌研究室,河南医科
• 出 处: 中国病理生理杂志
• 发表日期: 1989年03期
• 关键词: 交链 单甲 链格孢属 诱变性试验 食管肿瘤 致突变作用 食管癌 突变频率 提取物 溶剂对照

交链孢醇单甲醚是从林县污染粮食中分离的互隔交链孢霉的提取物,经进一步纯化结晶获得,它是该霉菌的主要代谢产物之一。本文用V_(79)细胞突变的方法进行了交链孢醇单甲醚的致突变性研究。结果表明,在不加S-9和加S-9情况下,交链孢醇单甲醚均能诱发V_(79)细胞6-巯基鸟嘌呤抗性突变,随交链孢醇单甲醚剂量增大,突变频率增高,具有直线回归关系(P<0.01),但不加S-9时比加S-9时致突变作用更强,提示交链孢醇单甲醚是直接致突变物,可能与食管癌病因有一定关系。

It is one of the main metabolites of this mold, which is obtained from further purification and crystallization of isolates of Alternaria alternata separated from contaminated grain in Linxian County. In this paper, mutagenesis of V_ (79) cells was used to study the mutagenicity of streptolol monomethyl ether. The results showed that streptomycin monomethyl ether could induce 6-mercap guanine resistance mutation in V 79 cells without adding S-9 and S-9, (P <0.01). However, S-9 was more effective than S-9 in mutagenicity, suggesting that streptomycin monomethyl ether was a direct mutagen, May have a certain relationship with the cause of esophageal cancer.