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用于叶期苗在不接种病毒条件下测试过氧化物酶同工酶结果,在相对迁移率17％—47％内,有6个位点出现酶带,抗、感病品种的酶带数量、酶带出现的位点和强弱程度有明显差别。高抗品种酶带数最多,有E_3和E_4强带,E_3为弱带;高感品种酶带数最少,缺E_6和E_4,E_3为强带;低抗和低感品种的酶带介于高抗与高感品种之间。用酶带数量和各酶带强弱程度,或用酶谱薄层扫描积分值计算距离系数,而后聚类,聚类结果与抗、感病性分组完全一致。证明抗病性与过氧化物酶同工酶有密切关系,用不接种苗酶谱测定可以代替接种鉴定和田间鉴定。
For the leaves of seedlings without peritoneal in the test of the peroxidase isozyme results, in the relative mobility of 17% -47%, there are six loci, anti-susceptible susceptible varieties of the number of bands , There is a significant difference between the location of the enzyme band and the degree of strength. The bands of high resistant varieties had the largest number of bands with E_3 and E_4 strong bands and E_3 weak bands, the least susceptible bands with less E_6 and E_4, E_3 strong bands, and those with low and low susceptible bands Between anti-and high-susceptible varieties. The distance coefficient was calculated by the number of enzyme bands and the intensity of each enzyme band, or the integral value of the zymogram of zymogram, and then the clustering results were completely consistent with the resistance and susceptibility groups. Prove the disease resistance and peroxidase isozymes are closely related with non-inoculation seedling zymogram determination can replace the inoculation and field identification.