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作者用 DNA 流式细胞光度测定法检查精液中的非整倍体细胞,以期建立一种无创伤性的诊断睾丸原位癌的方法。8名经睾丸活检证实的睾丸原位癌病人,以及26名没有证据表明有睾丸肿瘤的对照者接受此项检查。前者在治疗之前取射精精液标本检查,其中4人在治疗后(睾丸切除或局部放疗)一年取射精精液标本复查。方法:待精液液化后,取部分标本用0.9%氯化钠洗涤一次,在1M 蔗糖溶液中离心。
The authors used DNA flow cytometry to examine aneuploid somatic cells in semen to establish a non-invasive method for the diagnosis of testicular carcinoma in situ. Eight patients with testicular carcinoma confirmed by testicular biopsy and 26 controls without evidence of testicular tumor underwent this test. The former was taken before the treatment of sperm injection sperm samples, of which 4 after treatment (orchiectomy or local radiotherapy) a year to take sperm samples review. Methods: After seminal fluid liquefaction, take part of the samples with 0.9% sodium chloride once, centrifuged in 1M sucrose solution.