Extraction and Absolute Crystal Structure of Stachyose

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The title compound stachyose(C24H42O21),a biologicaly active tetrasaccharide,was characterized by X-ray diffraction analysis.It crystallizes in the orthorhombic system,space group P21212 with C24H42O21,a = 23.8760(3),b = 12.71028(12),c = 10.81279(11) ,V = 3281.36(6) 3,Z = 4,Dc = 1.511 g/cm3,Mr = 746.58,F(000) = 1576,and μ = 1.230 mm-1.The final R = 0.0666 and wR = 0.1797 for 6298 observed reflections(I > 2σ(I)).The molecular crystal structure of stachyose shows absolute stereochemistry of β-D-fructofuranosyl α-D-galactopyranosyl-(1→6)-α-D-galactopyranosyl-(1→6)-α-D-glucopyranoside.The molecule is composed of two α-D-galactoses,one α-D-glucose,and one β-D-fructose and sequentially linked as α-Gal(1→6) α-Gal(1→6) α-Glc(1→2) β-Fru.The title compound is stacked into a 3D layer structure through hydrogen bonds.NMR spectra data are also assigned.In the crystal packing,X-ray analysis indicates that there are two intramolecular and eleven intermolecular hydrogen bonds in this compound. The title compound stachyose (C24H42O21), a biological active tetrasaccharide, was characterized by X-ray diffraction analysis. It crystallizes in the orthorhombic system, space group P21212 with C24H42O21, a = 23.8760 (3), b = 12.71028 = 10.81279 11, V = 3281.36 63, Z = 4, Dc = 1.511 g / cm3, Mr = 746.58, F (000) = 1576 and μ = 1.230 mm-1.The final R = 0.0666 and wR = 0.1797 for 6298 observed reflections (I> 2σ (I)). The molecular crystal structure of stachyose shows absolute stereochemistry of β- D-fructofuranosyl α-D-galactopyranosyl- (1 → 6) -α-D-galactopyranosyl - (1 → 6) -α-D-glucopyranoside. The molecule is composed of two α-D-galactoses, one α-D-glucose, and one β-D-fructose and sequentially linked as α-Gal ) α-Gal (1 → 6) α-Glc (1 → 2) β-Fru. The title compound is stacked into a 3D layer structure through hydrogen bonds.NMR spectra data are also assigned to the crystal packing, X-ray analysis indicates that there are two intramolecular and eleven intermolecular hydrogen bo nds in this compound.
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