抗Fas单克隆抗体诱导人胃癌细胞系SGC-7901细胞

• 作 者: 刘海峰 刘为纹 房殿春
• 机 构: 第三军医大学西南医院消化科
• 出 处: 世界华人消化杂志
• 发表日期: 1999年06期
• 关键词: 人胃癌细胞系 单克隆抗体 SGC-7901细胞 胃肿瘤 胃癌治疗 细胞凋亡指数 流式细胞光度术 增殖周期 杀伤作用 细胞形态观察

目的探讨抗Ｆａｓ单克隆抗体诱导胃癌细胞凋亡的规律及在胃癌治疗中的意义．方法应用细胞形态观察、琼脂糖凝胶电泳、流式细胞光度术检测抗Ｆａｓ单克隆抗体对胃癌细胞ＳＧＣ７９０１增殖周期的影响以及对细胞杀伤作用的方式，并检测了ＳＧＣ７９０１细胞表面Ｂｃｌ２的表达情况．．结果抗Ｆａｓ单克隆抗体有阻滞细胞周期、通过诱发凋亡而抑制肿瘤细胞生长的作用．经抗Ｆａｓ单克隆抗体处理后，ＳＧＣ７９０１细胞表面Ｂｃｌ２蛋白表达无明显变化．．结论抗Ｆａｓ单克隆抗体可以诱导胃癌细胞系ＳＧＣ７９０１细胞凋亡．抗Ｆａｓ单克隆抗体诱导胃癌细胞凋亡与Ｂｃｌ２表达无关

Objective To investigate the regulation of gastric cancer cell apoptosis induced by anti-Fas monoclonal antibody and its significance in the treatment of gastric cancer. Methods Cellular morphology, agarose gel electrophoresis and flow cytometry were used to detect the effect of anti-Fas monoclonal antibody on the proliferation cycle of gastric cancer cell line SGC 7901 and its killing effect on cells. The cell surface Bcl of SGC7901 cells was also detected. The expression of _2. . RESULTS: Anti-Fas monoclonal antibody could block the cell cycle and inhibit the growth of tumor cells by inducing apoptosis. After treatment with anti-Fas monoclonal antibody, the expression of Bcl2 protein on the surface of SGC7901 cells had no significant changes. . Conclusion Anti-Fas monoclonal antibody can induce apoptosis of gastric cancer cell line SGC-7901. Anti-Fas monoclonal antibody induces apoptosis in gastric cancer cells and is not related to Bcl2 expression