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目的了解视力检查遮眼板的带菌情况及3种消毒剂消毒效果比较,探讨适宜的消毒方法。方法在消毒前使用500mg/L含氯消毒剂、75%乙醇、异丙醇复合消毒湿巾3种不同方法对遮眼板消毒处理后即刻采样、使用5、10人次后分别采样,进行细菌学检测,计数菌落数并进行细菌鉴定,检测结果进行统计分析。结果未经消毒的遮眼板带菌率100.00%,主要病原菌为金黄色葡萄球菌、铜绿假单胞菌及变形菌属;分别用500mg/L含氯消毒剂、75%乙醇、异丙醇复合消毒湿巾3种方法消毒后即刻采样结果,细菌总数均减少,杀菌率分别为99.46%、99.46%、99.89%,与消毒前相比,差异有统计学意义(P<0.01);使用5、10人次后采样检测结果显示,异丙醇复合消毒湿巾的消毒持续效果优于含氯消毒剂及乙醇(P<0.01或P<0.05)。结论视力检查遮眼板带菌情况严重,应进行消毒处理,异丙醇复合消毒湿巾、500mg/L含氯消毒剂消毒效果优于75%乙醇。
OBJECTIVE To understand the carrying status of ophthalmic examination and the disinfection effect of three kinds of disinfectants and to explore the suitable disinfection methods. Methods Three different methods, including disinfecting 500mg / L chlorinated disinfectant, 75% ethanol and isopropanol compound disinfecting wipes, were used to sample the obscured leaves immediately after disinfection. After 5 and 10 times of disinfection, samples were taken for bacteriology Detection, counting the number of colonies and bacteria identification, test results for statistical analysis. Results The non-disinfected ophthalmic tape was 100.00%, the main pathogens were Staphylococcus aureus, Pseudomonas aeruginosa and Proteus. The disinfectants were disinfected with 500 mg / L chlorine disinfectant, 75% ethanol and isopropanol respectively The results showed that the total number of bacteria decreased after disinfection, the total number of bacteria were all decreased, the rate of sterilization was 99.46%, 99.46%, 99.89% respectively. There was significant difference between before and after disinfection (P <0.01) After sampling, the results showed that the continuous disinfection of isopropyl alcohol compound disinfection wipes better than chlorine disinfectant and ethanol (P <0.01 or P <0.05). CONCLUSION: The vaginal discharge of eyesight examination is serious and should be disinfected. Isopropyl alcohol disinfectant wipes and disinfectant with 500mg / L chlorine disinfectant are better than 75% ethanol.