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本文报道用Cetrimide~(R)(溴化十六烷三甲铵)治疗动物和人的细粒棘球蚴囊的体外,体内试验评价研究。作者从牛和羊的肺及肝内获得新鲜的棘球蚴囊,有些蚴囊的原头蚴按Meymarian等(1963)的方法收集。并用生理盐水悬浮及沉淀洗涤3次,然后取1滴约含500个原头蚴的悬浮液分别加至4支盛装2ml Cetrimi-de~(R)溶液(浓度为0.05%、0.1%、0.5%和1%)的试管中充分混匀,保温在37℃水浴中,孵育10,5和1分钟后,再用生理盐水悬浮沉淀洗涤3次,保温在37℃中约30分钟。
This paper reports the in vitro and in vivo evaluation of Cetrimide R (hexadecane trimethylammonium bromide) in the treatment of Echinococcus granulosus in animals and humans. The authors obtained fresh hydatid cysts from the lungs and liver of cattle and sheep, some of which are collected according to the method of Meymarian et al. (1963). And washed three times with physiological saline suspension and sediment, and then one drop of suspension containing about 500 protoscoleces was added to 4 tubes containing 2 ml Cetrimi-de R solution (concentration of 0.05%, 0.1%, 0.5% And 1%). The tubes were incubated in a water bath at 37 ° C for 10, 5 and 1 minutes before being washed three times with physiological saline suspension pellet and incubated at 37 ° C for about 30 minutes.