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20例胚胎胰腺、5例胰腺癌、4例正常胰腺提取物行聚丙烯酰胺凝胶电泳(SDS-PAGE)、单克隆抗体J28免疫印渍和花生凝集素印渍,结果发现考马斯亮蓝染色后胚胎胰腺和胰腺癌提取物有一相同条带,分子量约为110000,这一条带即是胚胰腺泡蛋白(FAP,它可与单克隆抗体J28和花生凝集素结合,正常胰腺无此条带。花生凝集素还可以和胰腺癌组织中分子量更小的糖蛋白结合,说明FAP分子上有单克隆抗体J28和花生凝集素的不同位点。
20 cases of embryonic pancreas, 5 cases of pancreatic cancer, 4 cases of normal pancreas extract by polyacrylamide gel electrophoresis (SDS-PAGE), monoclonal antibody J28 immunoblot and peanut agglutinin staining, found Coomassie brilliant blue staining An identical band of embryonic pancreas and pancreatic cancer extract with a molecular weight of about 110,000 is the embryonic pancreatin protein (FAP), which binds to the monoclonal antibody J28 and peanut agglutinin but not to the normal pancreas. Lectins can also bind to smaller molecular weight glycoproteins in pancreatic cancer tissue, indicating that FAP molecules have different sites on the monoclonal antibody J28 and peanut lectin.