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冰冻切片质量的好坏直接关系到能否准确及时地作出病理诊断,以指导手术治疗方式,近年来,国内外学者对如何提高冻冻切片及染色质量做了大量工作,但仍然存在着组织结构被冰晶破坏,对比染色差,细胞核着色不清晰等缺点,我们应用微波加热固定液对组织块进行预固定,切片后再次固定的“二次固定技术”处理肿瘤组织,获得了比单纯脱脂固定切片更好的效果.1 材料与方法1.1 材料 选用我院1994～1995年外科送检需要急诊冰冻的乳腺、胃、肠、卵巢、甲状腺及脑等处的肿瘤组织,取材2cm×1.5cm×0.5cm数块.YWY781A型医用微波炉为浙江临安爱迪电子仪器厂产品,有8个功率选择开关,最大输出功率为500W.1.2 方法(1)将肿瘤组织分为不处理和处理组,处理组分别用生理盐水,10%甲醛和Bouin液在80℃恒温水浴固定5min,用微波2档(输出功率250W)固定1min.(2)将上述肿瘤组织用恒冷切片机切成5μm厚的冰冻切片数张,贴片稍于后分成不固定组和固定组,固定组分别用酒精-冰醋酸-甲醛固定液(AAF).改良的
The quality of frozen section is directly related to the accuracy and timely pathological diagnosis to guide the surgical treatment. In recent years, domestic and foreign scholars have done a lot of work on how to improve the quality of frozen section and staining, but there is still an organizational structure. Due to defects such as ice crystal destruction, poor contrast staining, and unclear cell nuclear staining, we applied microwave heating fixative solution to pre-fix the tissue block, and then fixed the “secondary fixation technique” to treat the tumor tissue after slicing, and obtained a fixed slice than simply defatted. Better results. 1 Materials and methods 1.1 Materials used in our hospital from 1994 to 1995 for emergency examination of the need for emergency diagnosis of frozen breast, stomach, intestine, ovary, thyroid and brain, tumor tissue, drawn 2cm × 1.5cm × 0.5cm Several YWY781A medical microwave ovens are products of Zhejiang Lin’an Aidi Electronic Instrument Factory. There are 8 power selector switches and the maximum output power is 500W.1.2. Method (1) The tumor tissues are divided into non-treatment and treatment groups, and the treatment groups are used separately. Normal saline, 10% formalin and Bouin solution were fixed in a constant temperature water bath at 80°C for 5 min, and fixed with microwave 2nd gear (output power 250 W) for 1 min. (2) The above tumor tissue was frozen with a cryostat 5μm thick frozen sections as the number of sheets, a little after the patch is not fixed to the divided groups and the fixing group, fixing each group with alcohol - acetic acid - formaldehyde fixative (AAF) modified