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为弄清高Leptin水平在不同免疫状态下对免疫系统的影响 ,选用 8周龄的清洁级BALB/c雄性小鼠共 4 0只 ,分成五组 ,分别用外源性鼠Leptin和Leptin抗体人为形成小鼠的不同Leptin水平状态 ,并利用CsA将小鼠分为正常免疫和免疫抑制状态两类 ,两种状态各设对照组 ,测定细胞增殖情况 ;用ELISA方法检测外周血和细胞培养液中IL 2、IL 4、IFN γ和Leptin的浓度。正常免疫状态下 ,Leptin组的脾细胞增殖与对照组无差异 ,细胞因子除IFN γ升高外 ,IL 2、IL 4均无差异 ;Leptin抗体组显示脾细胞增殖能力下降 ,各细胞因子测定示显示差异 ;免疫抑制状态下 ,Leptin组的细胞增殖活力较强 ,IL 2、IFN γ水平上升 ,IL 4分泌降低 ,这种结果也在几乎所有体外加入Leptin的各组中出现。表明Leptin可以起免疫调节作用 ,增加IFN γ、IL 2的合成 ,降低IL 4的产生 ,使免疫应答向Th1细胞方向偏离。在正常免疫状态下Leptin的调节作用不明显 ,而在免疫抑制情况下 ,这种调节作用可以得到体现。
In order to find out the effect of high leptin level on the immune system under different immunological conditions, 40 clean BALB / c male mice of 8 weeks old were selected and divided into five groups. Human leptin and Leptin antibody The formation of different levels of Leptin mice, and the use of CsA mice were divided into two groups of normal immunity and immunosuppression, two states were set up control group, measured cell proliferation; ELISA method for detection of peripheral blood and cell culture medium IL 2, IL 4, IFN γ and Leptin concentrations. Under normal immune status, the proliferation of splenocytes in Leptin group was not different from that in control group. There was no difference in IL 2 and IL 4 except cytokines except IFNγ. Leptin antibody group showed decreased ability of proliferation of splenocytes, Showed a significant difference in immunosuppression state, Leptin group cell proliferation activity stronger, IL 2, IFN γ levels increased, IL 4 secretion decreased, this result also in almost all the addition of Leptin in each group appeared. Leptin can play an immunomodulatory role, increase IFN γ, IL 2 synthesis, reduce the production of IL 4, the immune response to Th1 cells in the direction of departure. The regulatory role of Leptin in normal immune status is not obvious, and in the case of immunosuppression, this regulatory effect can be reflected.