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为了探明影响水稻纹枯病菌营养体亲和性分化的因子,将水稻纹枯病菌菌株cx-2在不同水稻品种继代接种,并在含不同杀菌剂、具不同p H及不同温度条件的PDA平板上继代培养,用对峙法测定继代菌株与原始接种菌株的营养体亲和性。此外,对营养体亲和性分化菌株与原始菌株的AFLP指纹图谱进行比较。结果显示,在供试的30个水稻品种上连续接种4次后,从9个水稻品种中分离出与原始菌株营养体不亲和的菌株。菌株在不同p H值的PDA平板继代培养4次后,在p H偏碱性端(p H 10、p H 11)开始出现营养体亲和性分化的菌株。在不同农药和温度条件下继代培养10次的菌株中没有分离到营养体亲和性分化的菌株。营养体亲和性分化菌株与原始菌株的AFLP指纹图谱没有差异。
In order to find out the factors affecting the differentiation of Rhizoctonia solani to vegetative body, the rice sheath blight fungus strain cx-2 was inoculated on different rice varieties in succession and incubated in different fungicides with different p H and different temperature Conditioned PDA plates were subcultured and the tropism of the vegetative organisms of the subculturing strains and the original inoculated strains was determined by the confrontation method. In addition, AFLP fingerprinting of strains of vegetatively compatible differentiated strains was compared with that of the original strain. The results showed that after inoculating four times on 30 rice cultivars tested in succession, strains incompatible with the original vegetative bacteria were isolated from 9 rice cultivars. Strains were subcultured four times on PDA plates with different pH values and began to show vegetative affinity differentiation at the pH alkaline end (pH10, pH11). Under different pesticides and temperature conditions subculture 10 times in the strains did not separate the differentiation of vegetative bacteria strains. There was no difference in AFLP fingerprinting between vegetatively compatible strains and the original strain.