【摘 要】
：
AIM: To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation.METHODS: The expressions of cell adhesion molec
【机 构】
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Beijing Institute for Cancer Research and School of Oncology,Peking University,Beijing 100034,China
【基金项目】
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国家\"211\"工程建设项目;国家重点基础研究发展计划(973计划);国家自然科学基金
论文部分内容阅读
AIM: To explore the expression of cadherin isoforms in cultured human gastric carcinoma cells and its regulation.METHODS: The expressions of cell adhesion molecules (including E-cadherin, N-cadherin, α-catenin, β-catenin)and cadherin transcription factors including snail, slug and twist were determined by reverse transcriptasepolymerase chain reaction(RT-PCR), immunoblotting and immunofluorescence in SV40-immortalized human gastric cell line Ges-1 and human gastric cancer cell lines MGC-803, BGC-823 and SGC-7901.RESULTS: All cell lines expressed N-cadherin, but not E-cadherin. N-cadherin immunofluorescence was detected at cell membranous adherents junctions where co-localization with immunofluorescent staining of inner surface adhesion proteins α- and β-catenins was observed. The transformed Ges-1 and gastric cancer cell lines all expressed transcription factors (snail, slug and twist) which inhibited the expression of E-cadherin and triggered epithelial-mesenchymal transformation.CONCLUSION: Cadherin isoforms can change from E-cadherin to N-cadherin in transformed human gastric cancer cells, which is associated with intracellular events of stomach carcinogenesis and high expression of corresponding transcription factors.
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