论文部分内容阅读
逆转录聚合酶链式反应（Ｒｅｖｅｒｓｅ Ｔｒａｎｓｃｒｉｐｔｉｏｎ ａｎｄ Ｐｏｌｙｍ ｅｒａｓｅ Ｃｈａｉｎ Ｒｅａｃｔｉｏｎ，ＲＴ－ＰＣＲ）具有灵敏度高、准确性好的特点，因而被广泛应用于植物病毒检测．在ＲＴ反应中，ＲＮＡ 样品的快速制备尤为重要．用异丙醇二步沉淀法获得ＲＮＡ，快速检测水稻叶片中ＲＳｔＶ 的ＲＴ－ＰＣＲ 方法，可使测定时间缩短至６ ｈ，并可从０．０７８ｍ ｇ 感病叶片中检测出ＲＳｔＶ．这说明用异丙醇二步沉淀法提取的ＲＮＡ可以用作ＲＴ反应的模板
Reverse Transcription and Polym-erase Chain Reaction (RT-PCR) has been widely used in plant virus detection because of its high sensitivity and good accuracy. In RT reactions, rapid preparation of RNA samples is particularly important. RNA was obtained by two-step precipitation of isopropanol, and the RT-PCR method for rapid detection of RStV in rice leaves could shorten the assay time to 6 h and detect RStV from 0.078 m g of infected leaves. This shows that RNA extracted by isopropanol two-step precipitation can be used as a template for the RT reaction