Effects of Temperature, Acetate and Nitrate on Methane Generation from Petroleum Hydrocarbons

• 作 者: Liu Chunshuang Zhao Dongfeng Zhang Yunbo
• 机 构: College of Chemical Engineering, China University of Petroleum, Qingdao 266555
• 出 处: 中国炼油与石油化工（英文版）
• 发表日期: 2014年4期
• 关键词: petroleum hydrocarbons nitrate methane microbial community

In this study, the effects of temperature, acetate and nitrate on methane gas production from biodegradation of petroleum hydrocarbons were investigated. The results indicated that methane gas production at 35℃was higher than that obtained at 55℃. The acetate addition signiifcant enhanced the methane production at 35℃, however, at 55℃the nitrate addition could largely promote the methane production. The microbial community structures were revealed by PCR-DGGE. The Actinobacteria, Clostridia, Clostridiales, Syntrophus, Pseudomonas, and Proteobacteria-like bacteria and Methano-cellales, Methanosaeta, Methanomicrobiales, Methanolinea, Thermoprotei-like archaea had been enriched at 35 ℃in the acetate addition test. The Thermoprotei, Proteobacterium, Thermodesulfovibrio-like bacteria and Methanocellales-like ar-chaea had been enriched at 55℃in the nitrate addition test. The results may shed light on the bio-utilization of marginal oil reservoirs for enhancing energy recovery.