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本实验旨在研究取自不同胎龄的大鼠胎儿器官组织植入同种大鼠体内的效果。用杂交系Sprague-Dawley大鼠,受体为10周龄雌性大鼠,重225～250 g。怀孕11～13天的大鼠选为供体。移植组织:成熟大鼠肾组织(组Ⅰ)的采取,系切除双侧肾脏,立即置于改良的Eagle培养液中(4℃),在解剖显微镜下切成直径小于1 mm的组织块。胎肾组织(组Ⅱ)、胎性腺组织(组Ⅲ)和胎肝组织(组Ⅳ)的采取,是在大鼠受孕后第15～21天,分别剖腹切开子宫取胎,摘取胎儿肝、肾和性腺组织,置于上述培养液中,切成同样大小碎片,此外还采取胎龄为13天的胎肝。采用肾包膜下埋藏技术,将组织移植于受体之左肾包膜下的二个不同部
The purpose of this study was to investigate the effects of implantation of fetal organ tissue from rats of different gestational age into the same rat. Sprague-Dawley rats were used as the recipients, 10-week-old female rats weighing 225-250 g. Rats 11 to 13 days pregnant were selected as donors. Transplanted Tissue: Mature rat kidney tissue (Group I) was taken and both kidneys were excised and immediately placed in a modified Eagle’s medium (4 ° C) and dissected into tissue blocks less than 1 mm in diameter under a dissecting microscope. Fetal kidney tissue (group Ⅱ), fetal gonadal tissue (group Ⅲ) and fetal liver tissue (group Ⅳ) were taken from the 15th to the 21th day after conception in rats. , Kidneys and gonadal tissues were placed in the above culture medium, cut into the same size of debris, in addition to fetal gestational age was 13 days of fetal liver. The renal capsule under the buried technology, the organization transplanted in the recipient of the left renal capsule under the two different ministries