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目的探讨晚期非小细胞肺癌患者外周血CD4~+ CD25~+ FOXP3~+ 调节性T(Treg)细胞的表达及其临床意义。方法采用免疫荧光术及流式细胞仪检测50例晚期非小细胞肺癌患者及50例健康对照组外周血中CD4~+ CD25~+ FOXP3~+ Treg细胞、CD4~+ CD25~+ Treg细胞、CD4~+ T细胞和CD4~+ CTLA-4~+ T细胞的表达。结果晚期非小细胞肺癌患者外周血中CD4~+ CD25~+ FOXP3~+ Treg细胞、CD4~+ CD25~+ Treg细胞和CD4~+ CTLA-4~+ T细胞的比例均高于健康对照组(均P<0.05),CD4~+ T细胞的比例均低于健康对照组(均P<0.05)。结论晚期非小细胞肺癌患者外周血CD4~+ CD25~+ FOXP3~+ Treg细胞比例高于健康对照者,可能与肺癌患者的免疫抑制和肿瘤进展相关。
Objective To investigate the expression of CD4 ~ + CD25 ~ + FOXP3 ~ + regulatory T (Treg) cells in peripheral blood of patients with advanced non-small cell lung cancer and its clinical significance. Methods The levels of CD4 ~ + CD25 ~ + FOXP3 ~ + Treg cells, CD4 ~ + CD25 ~ + Treg cells, CD4 ~ + CD25 ~ + Treg cells in peripheral blood of 50 patients with advanced non-small cell lung cancer and 50 healthy controls were detected by immunofluorescence and flow cytometry ~ + T cells and CD4 ~ + CTLA-4 ~ + T cells. Results The proportion of CD4 ~ + CD25 ~ + FOXP3 ~ + Treg cells, CD4 ~ + CD25 ~ + Treg cells and CD4 ~ + CTLA-4 ~ + T cells in peripheral blood of patients with advanced non-small cell lung cancer were significantly higher than those of healthy controls All P <0.05), and the percentage of CD4 ~ + T cells were lower than those in healthy controls (all P <0.05). Conclusion The proportion of CD4 ~ + CD25 ~ + FOXP3 ~ + Treg cells in patients with advanced non-small cell lung cancer is higher than that of healthy controls, which may be related to the immunosuppression and tumor progression in patients with lung cancer.