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采用有机改性溶胶凝胶-聚乙烯醇(Ormosil-PVA)包埋葡萄糖氧化酶,制备普鲁士蓝修饰玻碳电极,并基于此制备了安培型葡萄糖生物传感器。以含有FeCl3,K3Fe(CN)6和EDTA的溶液为沉积液,通过循环伏安电沉积法制备了高稳定性的普鲁士蓝修饰电极。考察了扫描电位范围、外加电位、pH和温度等因素对传感器的影响。葡萄糖浓度在20μmol/L~2 mmol/L范围内与响应电流呈线性关系,相关系数R=0.9965,以3倍空白值的标准偏差计算此传感器的检出限为8.1μmol/L。其中,在低浓度(20~100μmol/L)范围内,电流与浓度也呈良好的线性关系(R=0.9938)。
Glucose oxidase was embedded in organic modified sol-gel-polyvinyl alcohol (Ormosil-PVA), Prussian blue modified glassy carbon electrode was prepared, and ampicillin biosensor was prepared. A high stability Prussian blue modified electrode was prepared by cyclic voltammetry with a solution containing FeCl3, K3Fe (CN) 6 and EDTA as the deposition solution. The influence of scanning potential range, applied potential, pH and temperature on the sensor was investigated. The linear relationship between the concentration of glucose and the response current in the range of 20μmol / L ~ 2mmol / L was obtained. The correlation coefficient R was 0.9965. The detection limit of this sensor was 8.1μmol / L with the standard deviation of 3 times blank value. Among them, there was a good linear relationship between current and concentration at low concentration (20-100μmol / L) (R = 0.9938).