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目的探讨续断抗衰老作用的机理。方法用续断（２ｇ／ｄ／只）对ＡＤ模型大鼠灌胃治疗３个月。通过行为测试，光镜免疫细胞化学ＡＢＣ法，对ＡＤ大鼠行为改变，海马结构齿状回和ＣＡ１区ＡＰ－Ｌｉ神经元的形态和数目，β－ＡＰ的沉积变化进行了观察。结果续断组大鼠较对照组受电击次数减少，潜伏期延长（Ｐ＜０．０１），海马结构齿状回和ＣＡ１区ＡＰ－Ｌｉ神经元的数目较对照组均有明显减少（Ｐ＜０．０１），β－ＡＰ样反应染色减少。结论续断可以恢复ＡＤ模型大鼠的学习记忆缺损，有抑制和清除海马结构齿状回和ＣＡ１区β－ＡＰ的沉积的作用。
Objective To explore the mechanism of severed anti-aging effect. Methods The rats of AD model were intragastrically treated for 3 months with 2g / d per mouse. Behavioral tests and light microscopy immunocytochemical ABC method were used to observe the behavior of AD rats, the morphology and number of AP-Li neurons in dentate gyrus and CA1 area, and the deposition of β-AP in AD rats. Results Compared with the control group, the number of shocks and the latency of hippocampal neurons were decreased (P <0.01), the numbers of AP-Li neurons in hippocampus dentate gyrus and CA1 area were significantly decreased .01), β-AP-like reaction decreased staining. Conclusions Dipsacus can restore learning and memory deficits in AD rats, and inhibit and eliminate the deposition of β-AP in dentate gyrus and CA1 area of hippocampus.