论文部分内容阅读
目的探讨内生菌糖抗电磁辐射的生物学效应。方法建立人工磁场辐射装置;取健康人淋巴细胞,标明实验与对照;实验组加入细胞保护剂——内生菌糖,对照组加入同等体积的生理盐水;一并置于电磁场环境中培养观察;采用KL型免疫图像分析系统分别检测T淋巴细胞Ag-NORs I/S(核仁/核)面积之比。结果实验组T淋巴细胞Ag-NORs I/S 6.72±0.83%,高于对照组(5.81±1.28%),差别极为显著(P<0.01)。结论电磁辐射能降低人体T淋巴细胞Ag-NORs转录活性;内生菌糖能增强细胞的抗辐射能力。
Objective To investigate the biological effects of endophytic saccharide against electromagnetic radiation. Methods The artificial magnetic field radiation device was established. The healthy human lymphocytes were labeled with the experimental and control groups. The experimental group was treated with cytoprotective agent endosomic glucose and the control group with the same volume of physiological saline. The cells were placed under electromagnetic field for culture observation. The ratio of Ag-NORs I / S (nucleolus / nucleus) area of T lymphocytes was detected by KL immunoassay system. Results The Ag-NORs I / S of experimental group was 6.72 ± 0.83%, which was significantly higher than that of control group (5.81 ± 1.28%) (P <0.01). Conclusion Electromagnetic radiation can reduce the transcription activity of Ag-NORs in human T lymphocytes; endophytic saccharide can enhance the cell’s anti-radiation ability.