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AIM:To elucidate the distinctive pathobiological behaviorbetween signet ring cell carcinoma(SRC)and mucinousadenocarcinoma of the stomach.METHODS:Based on the histological growth patterns andcell-functional differentiation classifications of stomachcarcinoma,we conducted a series of comparative studies.All paraffin-embedded and frozen blocks were collected fromthe files of Cancer Institute of China Medical University.Onthe basis of histopathological observation,we appliedenzymatic and mucous histochemistry,immunohistochemistry,flow cytometry(FCM)and molecular biology to comparethese two categories of gastric cancers in terms of the DNAploidy,proliferative kinetics,the expression of gastriccarcinoma associated gene product and instabilities ofmitochondrial DNA(mtDNA).RESULTS:Gastric SRC was commonly seen in females below45 years,mostly presenting diffuse growth and ovary oruterine cervix metastasis.The majority of SRC wereabsorptive and mucus-producing functional differentiationtype(AMPFDT),which growth relied on estrogen.Meanwhile,stomach mucinous adenocarcinomas were mostly observedin males over 50 years,prone to massive growth or nestgrowth and extensive peritoneal infiltration,showing twocategories of cell-functional differentiation types:AMPFDTand mucus-secreting functional differentiation type(MSFDT).Expressions of ER,enzyme c-PDE and 67kDaLN-R in SRCwere evidently higher than that in mucinous adenocarcinoma,while expressions of LN,CN-IV,CD44v6,and PTEN proteinwere obviously lower in SRC than that in mucinousadenocarcinoma(P<0.05).There was no statistic significancein VEGF,ECD and instabilities of mtDNA(P>0.05)betweenthe above two gastric carcinomas.CONCLUSION:Though SRC and mucinous adenocarcinomawere both characterized by abundant mucus-secretion,theywere quite different in morphology,ultrastructure,cell-functional differentiation and protein expression,indicatingdifferent mechanisms of carcinogenesis.We concluded that combining histological growth patterns,cell-functionaldifferentiation type with tumor related markers might besignificant in early diagnosis and prognosis assessment forSRC and mucinous adenocarcinoma of the stomach.
AIM: To elucidate the distinctive pathobiological behaviorbetween signet ring cell carcinoma (SRC) and mucinousadenocarcinoma of the stomach. METHODS: Based on the histological growth patterns and cell-functional differentiation classifications of stomach carcinoma, we conducted a series of comparative studies. All paraffin-embedded and frozen blocks were collected from the files of Cancer Institute of China Medical University. Inthe basis of histopathological observation, we appliedenzymatic and mucous histochemistry, immunohistochemistry, flow cytometry (FCM) and molecular biology to compare the two two categories of gastric cancers in terms of the DNAploidy, proliferative kinetics, the expression of gastric cancer associated gene product and instabilities of mitochondrial DNA (mtDNA) .RESULTS: Gastric SRC was generally seen in females below45 years, mostly presenting diffuse growth and ovary oruterine cervix metastasis. the majority of SRC wereabsorptive and mucus-producing functional differentiationtype (AMP FDT), which growth relied on estrogen.Meanwhile, stomach mucinous adenocarcinomas were mostly observed in males over 50 years, prone to massive growth or nestgrowth and extensive peritoneal infiltration, showing twocategories of cell-functional differentiation types: AMPFDT and mucus-secreting functional differentiation types Expressions of ER, enzyme c-PDE and 67 kDaLN-R in SRCwere evidently higher than that in mucinous adenocarcinoma, while expressions of LN, CN-IV, CD44v6, and PTEN proteinwere obviously lower in SRC than that in mucinousadenocarcinoma (P < 0.05). There was no statistic significance in VEGF, ECD and instabilities of mtDNA (P> 0.05) betweenthe above two gastric carcinomas. CONCLUSION: Although SRC and mucinous adenocarcinomawere both characterized by abundant mucus-secretion, theywe quite different in morphology, ultrastructure, cell -functional differentiation and protein expression, indicating different mechanisms of carcinogenesis. We noted that combining histological growth patterns,cell-functional differentiation type with tumor related markers might besignificant in early diagnosis and prognosis assessment for SRC and mucinous adenocarcinoma of the stomach.