论文部分内容阅读
AIM:To prepare the human hepatocellular carcinoma-(HCC)-targeted liposome microbubbles and to investigate theirimmunological properties.METHODS:Human hepatocarcinoma specific monoclonalantibody HAb18 was attached to the surface of home-madelipasome microbubbles by static attraction to prepare thetargeted lipasome microbubbles.The combination of HAb18with liposome microbubbles was confirmed by the slideagglutination test and immunofluorescent assay.Theirimmunological activity was measured by ELISA.Rosetteformation test,rosette formation blocking test and immun-ofluorescent assay were used to identify the spedfic bindingof targeted liposome microbubbles to SMMC-7721 hepatomacells,and cytotoxicity assay was used to detect their effecton human hepatocytes.RESULTS:The targeted liposome microbubbles werepositive in the slide agglutinaUon test and immunofluorescentassay.ELISA indicated that the immunological activity ofHAb18 on the liposome microbubbles was similar to thatof free HAb18,SMMC-7721 cells were surrounded by thetargeting liposome microbubbles to form rosettes,whilethe control SGC-7901 gastric cancer ceils were not.Proliferationof SMMC-7721 cells and normal human hepatocytes wasnot influenced by the targeted liposome microbubbles.CONCLUSION:The targeted liposome microbubbles witha high specific biological activity have been successfullyprepared,which specifically bind to human hepatocarcinomacells,and are non-cytotoxic to hepatocytes.These resultsindicate that the liposome microbubbles can be used as aHCC-targeted ultrasound contrast agent that may enhanceultrasound images and thus improve the diagnosis of HCC,especially at the early stage.
AIM: To prepare the human hepatocellular carcinoma- (HCC) -targeted liposome microbubbles and to investigate theirimmunological properties. METHODS: Human hepatocarcinoma specific monoclonalantibody HAb18 was attached to the surface of home-made lipasome microbubbles by static attraction to prepare thetargeted lipasome microbubbles. The combination of HAb18 with liposome microbubbles was confirmed by the slideagglutination test and immunofluorescent assay. The immunohistological activity was measured by ELISA. Rosette formation test and rosette-blocking assay were used to identify the spedfic bindingof targeted liposome microbubbles to SMMC-7721 hepatoma cells, and cytotoxicity assay was used to detect their effecton human hepatocytes .RESULTS: The targeted liposome microbubbles werepositive in the slide agglutinaUon test and immunofluorescentassay. ELISA indicates that the immunological activity ofHAb18 on the liposome microbubbles was similar to that of free HAb18, SMMC-7 721 cells were surrounded by the targeting liposome microbubbles to form rosettes, while the control SGC-7901 gastric cancer ceils were not. Proliferation of SMMC-7721 cells and normal human hepatocytes was not influenced by the targeted liposome microbubbles. CONCLUSION: The targeted liposome microbubbles witha high specific biological activity have been successfullyprepared, which specifically bind to human hepatocarcinoma cells, and are non-cytotoxic to hepatocytes. These resultsindicate that the liposome microbubbles can be used as a HCC-targeted ultrasound contrast agent that may enhanceultrasound images and thus improve the diagnosis of HCC, especially at the early stage.