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Pre-harvest sprouting(PHS) occurs frequently in most of the wheat cultivation area worldwide,which severely reduces yield and end-use quality,resulting in substantial economic loss. In this study,quantitative trait loci(QTL) for PHS resistance were mapped using an available high-density single nucleotide polymorphism(SNP) and simple sequence repeat(SSR) genetic linkage map developed from a 269 recombinant inbred lines(RILs) population of Yanda 1817×Beinong 6. Using phenotypic data on two locations(Beijing and Shijiazhuang,China) in two years(2012 and 2013 harvesting seasons),five QTLs,designated as QPhs.cau-3A.1,QPhs.cau-3A.2,QPhs.cau-5B,QPhs.cau-4A,and QPhs.cau-6A,for PHS(GP) were detected by inclusive composite interval mapping(ICIM)(LOD≥2.5). Two major QTLs,QPhs.cau-3A.2 and QPhs.cau-5B,were mapped on 3AL and 5BS chromosome arms,explaining 6.29–21.65% and 4.36–5.94% of the phenotypic variance,respectively. Precise mapping and comparative genomic analysis revealed that the Ta Vp-1A flanking region on 3AL is responsible for QPhs.cau-3A.2. SNP markers flanking QPhs.cau-3A.2 genomic region were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection(MAS).
Pre-harvest sprouting (PHS) occurs frequently in most of the wheat cultivation area worldwide, which severely reduces yield and end-use quality, resulting in substantial economic loss. In this study, quantitative trait loci (QTL) for PHS resistance were mapped using an available high-density single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) genetic linkage map developed from a 269 recombinant in lines (RILs) population of Yanda 1817 × Beinong 6. Using phenotypic data on two locations (Beijing and Shijiazhuang, China) in two years (2012 and 2013 harvesting seasons), five QTLs, designated as QPhs.cau-3A.1, QPhs.cau-3A.2, QPhs.cau-5B, QPhs.cau- 4A, and QPhs.cau -6A for PHS (GP) were detected by inclusive composite interval mapping (ICIM) (LOD> 2.5). Two major QTLs, QPhs.cau-3A.2 and QPhs.cau-5B, were mapped on 3AL and 5BS chromosome arms , explaining 6.29-21.65% and 4.36-5.94% of the phenotypic variance, respectively. Precise mapping and comparative genomic analysis revealed that the Ta Vp-1A flanking region on 3AL is responsible for QPhs.cau-3A.2. SNP markers flanking QPhs.cau-3A.2 genomic regions were developed and could be used for introgression of PHS tolerance into high yielding wheat varieties through marker-assisted selection (MAS).