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本实验用无血清培养进行神经元与星形胶质细胞的联合培养，观察了星形胶质细胞和神经生长因子对新生大鼠基底神经节和下位脑干内的P物质受体阳性神经元存活与生长的影响。结果表明：（1）含神经生长因子的NeurobasalMedium／N2supplement（N／N2）能支持新生鼠脑神经元的存活和神经元突起的生长，其效果与加血清的Dulbacco改良Eagle培养基（s－DMEM）相似；（2）利用星形胶质细胞作为滋养细胞，种植新生鼠基底神经节和下位脑干细胞于其上，可见星形胶质细胞对神经元的突起生长（生长锥）有明显的促进作用，其中基底神经节神经元神经突的生长优于下位脑干神经元；（3）免疫组织化学反应结果显示，来源于基底神经节的培养神经元有87％为P物质受体阳性神经元，而下位脑干为84％，P物质受体阳性神经元的胞体形态完整，突起生长良好。本实验表明含神经生长因子的N／N2Medium或s－DMEM可作为P物质受体神经元体外培养的培养基，星形胶质细胞可在一定程度上支持新生大鼠脑P物质受体神经元的存活与突起生长，并可保持其分化特性。
In this experiment, we performed the co-culture of neurons and astrocytes with serum-free culture. The effects of astrocytes and nerve growth factor on the expression of substance P receptor positive neurons in the basal ganglia and inferior brainstem of neonatal rats The effects of survival and growth. The results showed that: (1) Neurobasal Medium / N2supplement (N / N2) containing nerve growth factor can support the survival of neurons and the neurite outgrowth in neonatal rats. The effect was similar to that of the serum-supplemented Dulbacco modified Eagle medium ). (2) Using astrocytes as feeder cells, newborn rat basal ganglia and inferior brain stem cells were planted on it, showing that astrocytes significantly promoted neurite outgrowth (growth cone) (3) The results of immunohistochemistry showed that 87% of cultured neurons derived from basal ganglia were P substance receptor positive neurons , While the lower brainstem was 84%. The morphology of neurons of substance P receptor positive cells was intact and the neurites grew well. This experiment shows that N / N2Medium containing nerve growth factor or s-DMEM can be used as medium for culturing P substance receptor neurons in vitro. Astrocytes can support the brain substance P receptor neurons The survival and protrusion of growth, and maintain their differentiation characteristics.